Yarlett, Nigel, Lloyd, David ORCID: https://orcid.org/0000-0002-5656-0571 and Williams, Alan G 1982. Respiration of the rumen ciliate Dasytricha ruminantium Schuberg. Biochemical Journal 206 (2) , 259--266. 10.1042/bj2060259 |
Abstract
The endogenous respiration of the rumen ciliate Dasytricha ruminantium maintained under an O2 tension of 2kPa (approximately 0.02 atm) was partially inhibited by KCN (40% inhibition) and NaN3 (58% inhibition). The organisms lack cytochromes, and sensitivity of respiration to KCN, NaN3, chloroquine and quercetin suggest that the operation of flavoprotein-iron-sulphur-mediated electron transport. As in Tritrichomonas foetus, hydrogenosomal respiration can be stimulated by the addition of CoA in the presence of 0.025% Triton X-100; stimulation by ADP was not detected. Stimulation of pyruvate-supported O2 uptake by Pi suggests that acetate is produced via acetyl phosphate.
Item Type: | Article |
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Date Type: | Publication |
Status: | Published |
Schools: | Biosciences |
Publisher: | Portland Press |
ISSN: | 0264-6021 |
Last Modified: | 26 Oct 2022 08:41 |
URI: | https://orca.cardiff.ac.uk/id/eprint/128060 |
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