Cardiff University | Prifysgol Caerdydd ORCA
Online Research @ Cardiff 
WelshClear Cookie - decide language by browser settings

The glutamate receptor?2 subunit controls post-synaptic density complexity and spine shape in the dentate gyrus

Medvedev, Nikolay I., Rodríguez-Arellano, José J., Popov, Victor I., Davies, Heather A., Tigaret, Cezar M. ORCID:, Schoepfer, Ralf and Stewart, Michael G. 2008. The glutamate receptor?2 subunit controls post-synaptic density complexity and spine shape in the dentate gyrus. European Journal of Neuroscience 27 (2) , pp. 315-325. 10.1111/j.1460-9568.2007.06005.x

Full text not available from this repository.


In adult brain the majority of AMPA glutamate receptor (GluR) subunits contain GluR2. In knock‐out (KO) mice the absence of GluR2 results in consequences for synaptic plasticity including cognitive impairments. Here the morphology of dendritic spines and their synaptic contacts was analysed via three‐dimensional reconstruction of serial electron micrographs from dentate gyrus (DG) of adult wild type (WT) and GluR2 KO mice. Pre‐embedding immunocytochemical staining was used to examine the distribution and subcellular localization of AMPA receptor GluR1 and N‐methyl‐d‐aspartate receptor NR1 subunits. There were no significant changes in synapse density in the DG of GluR2 KO compared with WT mice. However, in GluR2 KO mice there was a significant decrease in the percentage of synapses on mushroom spines but an increase in synapses on thin spines. There was also a large decrease in the proportion of synapses with complex perforated/segmented post‐synaptic densities (PSDs) (25 vs. 78% in WT) but an increase in synapses with macular PSDs (75 vs. 22%). These data were coupled in GluR2 KO mice with significant decreases in volume and surface area of mushroom spines and their PSDs. In both GluR2 KO and WT mice, NR1 and GluR1 receptors were present in dendrites and spines but there was a significant reduction in NR1 labelling of spine membranes and cytoplasm in GluR2 KO mice, and a small decrease in GluR1 immunolabelling in membranes and cytoplasm of spines in GluR2 KO compared with WT mice. Our data demonstrate that the absence of GluR2 has a significant effect on both DG synapse and spine cytoarchitecture and the expression of NR1 receptors.

Item Type: Article
Date Type: Publication
Status: Published
Schools: Medicine
Neuroscience and Mental Health Research Institute (NMHRI)
Publisher: Wiley
ISSN: 0953-816X
Date of First Compliant Deposit: 14 January 2020
Date of Acceptance: 20 November 2007
Last Modified: 26 Oct 2022 08:52

Citation Data

Cited 39 times in Scopus. View in Scopus. Powered By Scopus® Data

Actions (repository staff only)

Edit Item Edit Item