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Effect of blood volume on analytical bias in dried blood spots prepared for newborn screening external quality assurance

Moat, Stuart J, Dibden, Catherine, Tetlow, Lesley, Griffith, Caroline, Chilcott, Jim, George, Roanna, Hamilton, Laura, Wu, Teresa HY, MacKenzie, Finlay and Hall, Susan Kate 2020. Effect of blood volume on analytical bias in dried blood spots prepared for newborn screening external quality assurance. Bioanalysis 12 (2) , 99. 10.4155/bio-2019-0201

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Abstract

Aim: Dried blood spots (DBS) are used for the analysis of more than 2000 biomarkers. We assessed a range of analyte concentrations and diameters of DBS. Materials & methods: DBS samples were created by the application of increasing volumes of whole blood prepared by the UK NEQAS Quality Assurance Laboratory. Samples were analyzed in four separate laboratories. Results: Volumes less than 25 μl (8 mm) and more than 75 μl (14 mm) created unsatisfactory analytical biases. Results obtained from peripheral subpunches tended to be higher than those from a central subpunch. Conclusion: DBS diameters formed from nonvolumetric application of blood to filter paper can be used to assess whether measurement bias will be within acceptable limits according to the analyte being quantified. DBS received for newborn screening in the UK with diameters less than 8 mm and those more than 14 mm should be rejected.

Item Type: Article
Date Type: Publication
Status: Published
Schools: Medicine
Publisher: Future Science
ISSN: 1757-6180
Date of First Compliant Deposit: 27 February 2020
Date of Acceptance: 13 November 2019
Last Modified: 20 Jan 2021 09:30
URI: https://orca.cardiff.ac.uk/id/eprint/129989

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