Interrogating the osteogenic potential of implant surfaces in vitro: a review of current assays

Avery, Steven James, Ayre, Wayne Nishio ORCID: https://orcid.org/0000-0003-2405-1876, Sloan, Alastair James ORCID: https://orcid.org/0000-0002-1791-0903 and Waddington, Rachel Jane ORCID: https://orcid.org/0000-0001-5878-1434 2020. Interrogating the osteogenic potential of implant surfaces in vitro: a review of current assays. Tissue Engineering Part B: Reviews 26 (3) , pp. 217-229. 10.1089/ten.teb.2019.0312

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Abstract

The success of implantable devices relies heavily on their interaction with the host cells facilitating the osseointegration process. However, with so many new surface modifications, with subtly varying design parameters, in vitro assays can, with proper interpretation, provide valuable information for understanding cellular behavior. This review brings together pertinent in vitro experimental protocols available to researchers and discusses them in relationship to the development of the osteoblast phenotype during bone repair. Consideration is also paid to the influence of endothelial and macrophage cells that can substantially change osteogenic cell activity and thus can provide added value for predicting the osseointegration potential in vivo. Due to the diverse and heterogeneous nature of cell types available for culture use, this review concludes that there is no “gold standard” series of assays. Rather, we present guidance in the experimental design of in vitro assays to better identify those surfaces with promising osteogenic potential.

Item Type:	Article
Date Type:	Publication
Status:	Published
Schools:	Dentistry
Publisher:	Mary Ann Liebert, Inc.
ISSN:	1937-3368
Date of First Compliant Deposit:	9 March 2020
Date of Acceptance:	16 January 2020
Last Modified:	11 Nov 2024 01:30
URI:	https://orca.cardiff.ac.uk/id/eprint/130270

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