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A novel colorimetric assay for α-methylacyl-CoA racemase 1A (AMACR; P504S) utilizing the elimination of 2,4-dinitrophenolate

Yevglevskis, Maksims, Lee, Guat L., Nathubhai, Amit, Petrova, Yoana D., James, Tony D., Threadgill, Michael D., Woodman, Timothy J. and Lloyd, Matthew D. 2017. A novel colorimetric assay for α-methylacyl-CoA racemase 1A (AMACR; P504S) utilizing the elimination of 2,4-dinitrophenolate. Chemical Communications 53 (37) , pp. 5087-5090. 10.1039/C7CC00476A

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Abstract

α-Methylacyl-CoA racemase (AMACR; P504S) regulates branched-chain fatty acid degradation, activates Ibuprofen and is a recognised cancer drug target. A novel, facile colorimetric assay was developed based on elimination of 2,4-dinitrophenolate. The assay was used to test 5 known inhibitors, determining IC50 and Ki values, reversibility and characterizing irreversible inhibition.

Item Type: Article
Date Type: Publication
Status: Published
Schools: Chemistry
Publisher: Royal Society of Chemistry
ISSN: 1359-7345
Date of First Compliant Deposit: 13 July 2020
Date of Acceptance: 17 March 2017
Last Modified: 15 Jul 2021 01:11
URI: https://orca.cardiff.ac.uk/id/eprint/133378

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Cited 11 times in Scopus. View in Scopus. Powered By Scopus® Data

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