Catalytic production of hydrogen peroxide for in situ disinfection in medical applications

Suldecki, Grzegorz 2020. Catalytic production of hydrogen peroxide for in situ disinfection in medical applications. PhD Thesis, Cardiff University. Item availability restricted.

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Abstract

Contaminated endoscopes and infections associated with them have been repeatedly reported in the literature. There have been numerous outbreaks and pseudo-outbreaks associated with poorly disinfected endoscopes. Rinse water poses important problems in endoscope disinfection such as recontamination of endoscopes and contamination of patient samples. Biofilms are also a substantial problem for disinfection of endoscopes. The aim of this project is to explore an integrated system based on catalytic technology to produce H2O2 to provide sterile rinse water for endoscope reprocessing in automated endoscope reprocessors (AER). The catalytic technology used in this project was based on a gold and palladium catalyst which was tested in batch and flow reactors. Flow reactor treatment was a thousand times more effective at killing E. coli K12 JM109 (4 log10 reduction) than 200 ppm of commercial and batch reactor H2O2 in suspension (~1 log10 reduction). Moreover, flow reactor treatment with 1 w/w% AuPd/TiO2 catalyst was extremely effective against MS2 bacteriophages (8 log10 reduction) while 200 ppm of commercial and batch reactor H2O2 in suspension was ineffective (< 1 log10 reduction). Furthermore, 200 ppm of the flow reactor H2O2 prevented formation of E. coli K12 JM109 and B. subtilis ATCC6633 biofilms. H2O2 did not play a major role in the microbicidal activity of the catalyst. The proposed mechanism of microbicidal action is that in a H2/air mixture, H• initiates a reaction cascade which turns O2 into OOH• which can either attack the microorganisms on its own or can propagate a radical chain with contribution of the III H2O2 synthesised in the reactor which can support the flux of free radicals out of the surface of the catalyst. Ultimately, the system tested in this project has an innovative mechanism of action and showed a high microbicidal activity. However, further studies on its optimisation are necessary for its incorporation into AER.

Item Type:	Thesis (PhD)
Date Type:	Completion
Status:	Unpublished
Schools:	Pharmacy
Subjects:	Q Science > Q Science (General)
Date of First Compliant Deposit:	29 September 2020
Last Modified:	28 Sep 2021 01:30
URI:	https://orca.cardiff.ac.uk/id/eprint/135179

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