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Liposomes loaded with transforming growth factor β1 promote odontogenic differentiation of dental pulp stem cells

Jiang, Liming, Nishio Ayre, Wayne ORCID:, Melling, Genevieve E., Song, Bing ORCID:, Wei, Xiao-Qing ORCID:, Sloan, Alastair ORCID: and Chen, Xu 2020. Liposomes loaded with transforming growth factor β1 promote odontogenic differentiation of dental pulp stem cells. Journal of Dentistry 103 , 103501. 10.1016/j.jdent.2020.103501

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Objectives This study investigated whether novel liposome formulations loaded with transforming growth factor β1 (TGF-β1) could promote the odontogenic differentiation of human dental pulp stem cells (hDPSCs) for dentine-pulp regeneration. Methods 0-100 ng/mL of liposomal TGF-β1 was prepared using the thin-film hydration method. Release of TGF-β1 from the liposomes was quantified by an enzyme-linked immunosorbent assay (ELISA). The hDPSCs were treated with different concentrations of liposomal TGF-β1 and cell viability was tested using an MTT assay. “Osteodentine” differentiation capacity was assessed by RT-qPCR, ELISA and Alizarin red S staining. Results The ELISA results showed that liposomal TGF-β1 achieved a controlled and prolonged release over time. The MTT results demonstrated that the liposomes (100 μg/mL) were not cytotoxic to the cells. Liposomal TGF-β1 up-regulated the expression of “osteodentine” markers, RUNX-2, DMP-1 and DSPP, in hDPSCs after 7 days of treatment and resulted in the accumulation of mineralised nodules. Conclusion This study indicated that liposomes are an effective carrier for delivering TGF-β1 over time. Liposomal TGF-β1 promoted dentinogenesis and increased mineralisation in hDPSCs. This highlights the potential of liposomal TGF-β1 for future use in dentine-pulp regeneration. Clinical significance Liposomal TGF-β1 may be used as a synergist for promoting dentine-pulp regeneration of immature permanent teeth or as a pulp capping agent for inducing reparative dentine formation.

Item Type: Article
Date Type: Publication
Status: Published
Schools: Dentistry
Subjects: Q Science > Q Science (General)
R Medicine > RK Dentistry
Publisher: Elsevier
ISSN: 0300-5712
Funders: National Science Foundation of China (No. 81500898), Natural Science Foundation of Liaoning Province of China (No.2019-MS-387)
Date of First Compliant Deposit: 2 November 2020
Date of Acceptance: 1 October 2020
Last Modified: 23 Nov 2022 20:51

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