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Clonal relatedness and plasmid profiling of extensively drug-resistant New Delhi metallo-β-lactamase-producing Klebsiella pneumoniae clinical isolates

Qamar, Muhammad Usman, Ejaz, Hasan, Walsh, Timothy R. ORCID: https://orcid.org/0000-0003-4315-4096, Shah, Asad Ali, Al Farraj, Dunia A., Alkufeidy, Roua M., Alkubaisi, Noorah A., Saleem, Sidrah and Jahan, Shah 2021. Clonal relatedness and plasmid profiling of extensively drug-resistant New Delhi metallo-β-lactamase-producing Klebsiella pneumoniae clinical isolates. Future Microbiology 16 (4) , 229–239. 10.2217/fmb-2020-0315

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Abstract

Aim: Carbapenem-resistant Klebsiella pneumoniae (CR-KP) particularly New Delhi metallo-β-lactamase (NDM) is a serious public health concern globally. The aim of the study to determine the molecular epidemiology of blaNDM-producing clinically isolated K. pneumoniae. Methods: Carbapenem-resistant K. pneumoniae isolates (n = 100) were collected from tertiary care hospital Lahore. Isolates were confirmed by VITEK® 2 system and MALDI-TOF. Minimum inhibitory concentration was performed by VITEK 2 and molecular characterization was done by PCR, PFGE, DNA hybridization and replicon typing. Results: Of 90 MBL-producing K. pneumoniae, 75 were NDM producers; 60 were NDM-1 and 11 NDM-5. A total of 27 K. pneumoniae belonged to ST11 and 14 to ST147. NDM-positive isolates were 100% resistant to β-lactam antibiotics except for colistin. 13.3% isolates carried blaNDM on ∼140 kb plasmids. A total of 32 (52.4%) isolates were positive for IncA/C and 18 (29.5%) IncF/II. Conclusion: The extensively resistant lineage of NDM-producing K. pneumoniae is prevalent in the clinical setting.

Item Type: Article
Date Type: Published Online
Status: Published
Schools: Medicine
Publisher: Future Medicine
ISSN: 1746-0913
Last Modified: 09 Nov 2022 10:29
URI: https://orca.cardiff.ac.uk/id/eprint/139622

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