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Toxicity assessment of Dibutyl phthalate in Grass carp: an integrated biomarker approach

Faheem, Mehwish, Zahid, Zainab and Ferreira, Nuno G.C. ORCID: 2021. Toxicity assessment of Dibutyl phthalate in Grass carp: an integrated biomarker approach. Pakistan Veterinary Journal 41 (3) , pp. 365-371. 10.29261/pakvetj/2021.031

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Phthalates are the common plasticisers used around the globe. Dibutyl phthalate (DBP) is a ubiquitous, extensively used in cosmatics and frequently present in the aquatic environment. Therefore, toxic effects of DBP were evaluated in term of oxidative stress and biochemical biomarkers. For this reason, a 21 day exposure was conducted by exposing grass carp with graded concentrations of DBP (1, 10, 100 and 1000 µg/L). After 21days, stress biomarkers:, lipid peroxidation (LPO), catalase (CAT) activity, glutathione-S-transferases (GST) activity and level of reduced glutathione was evaluated in liver, kidney and gills. Alkaline phosphatase (ALP), aspartate transaminase (AST), urea and creatinine were evaluated in liver and kidney homogenates respectively. Moreover, effect of DBP on all biomarkers were evaluated through integrated biomarker response (IBR). Exposure of fish to DBP resulted in oxidative stress in grass carp as evidenced by an increase in lipid peroxidation and decrease in antioxidant enzymes. DBP exposure also resulted in increased liver’s ALT and AST levels. Urea and creatinine were also significantly increased in kidney after exposure to DBP. The IBR showed bad scores as the DBP concentration increased, with the highest one (1000 µg/L) presenting a score >250x the value for the control treatment. Additionally, the IBR/n showed that the most impacted organ was the kidney, followed by the liver and the gills. The obtained results show the need for deeper research into the effects of DBP on fish and their impact on different organs.

Item Type: Article
Date Type: Published Online
Status: Published
Schools: Biosciences
Subjects: Q Science > Q Science (General)
Q Science > QL Zoology
Publisher: University of Agriculture, Faisalabad
ISSN: 0253-8318
Date of First Compliant Deposit: 22 April 2021
Date of Acceptance: 7 March 2021
Last Modified: 11 Nov 2023 14:52

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