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Products of docosahexaenoate oxidation as contributors to photosensitising properties of retinal lipofuscin

Różanowska, Małgorzata B., Pawlak, Anna and Różanowski, Bartosz 2021. Products of docosahexaenoate oxidation as contributors to photosensitising properties of retinal lipofuscin. International Journal of Molecular Sciences 22 (7) , 3525. 10.3390/ijms22073525

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Abstract

Retinal lipofuscin which accumulates with age in the retinal pigment epithelium (RPE) is subjected to daily exposures to high fluxes of visible light and exhibits potent photosensitising properties; however, the molecules responsible for its photoreactivity remain unknown. Here, we demonstrate that autooxidation of docosahexaenoate (DHE) leads to the formation of products absorbing, in addition to UVB and UVA light, also visible light. The products of DHE oxidation exhibit potent photosensitising properties similar to photosensitising properties of lipofuscin, including generation of an excited triplet state with similar characteristics as the lipofuscin triplet state, and photosensitised formation of singlet oxygen and superoxide. The quantum yields of singlet oxygen and superoxide generation by oxidised DHE photoexcited with visible light are 2.4- and 3.6-fold higher, respectively, than for lipofuscin, which is consistent with the fact that lipofuscin contains some chromophores which do contribute to the absorption of light but not so much to its photosensitising properties. Importantly, the wavelength dependence of photooxidation induced by DHE oxidation products normalised to equal numbers of incident photons is also similar to that of lipofuscin—it steeply increases with decreasing wavelength. Altogether, our results demonstrate that products of DHE oxidation include potent photosensitiser(s) which are likely to contribute to lipofuscin photoreactivity.

Item Type: Article
Date Type: Publication
Status: Published
Schools: Optometry and Vision Sciences
Publisher: MDPI
ISSN: 1422-0067
Funders: Wellcome Trust
Date of First Compliant Deposit: 10 May 2021
Date of Acceptance: 25 March 2021
Last Modified: 24 May 2021 11:50
URI: https://orca.cardiff.ac.uk/id/eprint/141171

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