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Development of a high sensitivity ELISA detecting IgG, A & M antibodies to the SARS‐CoV‐2 spike glycoprotein in serum and saliva

Faustini, Sian E., Jossi, Sian E., Perez-Toledo, Marisol, Shields, Adrian M., Allen, Joel D., Watanabe, Yasunori, Newby, Maddy L., Cook, Alex, Willcox, Carrie R., Salim, Mahboob, Goodall, Margaret, Heaney, Jennifer L., Marcial-Juarez, Edith, Morley, Gabriella L., Torlinska, Barbara, Wraith, David C., Veenith, Tonny V., Harding, Stephen, Jolles, Stephen, Ponsford, Mark J., Plant, Tim, Huissoon, Aarnoud, O'Shea, Matthew K., Willcox, Benjamin E., Drayson, Mark T., Crispin, Max, Cunningham, Adam F. and Richter, Alex G. 2021. Development of a high sensitivity ELISA detecting IgG, A & M antibodies to the SARS‐CoV‐2 spike glycoprotein in serum and saliva. Immunology 164 (1) , pp. 135-147. 10.1111/imm.13349

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Detecting antibody responses during and after SARS‐CoV‐2 infection is essential in determining the seroepidemiology of the virus and the potential role of antibody in disease. Scalable, sensitive and specific serological assays are essential to this process. The detection of antibody in hospitalized patients with severe disease has proven relatively straightforward; detecting responses in subjects with mild disease and asymptomatic infections has proven less reliable. We hypothesized that the suboptimal sensitivity of antibody assays and the compartmentalization of the antibody response may contribute to this effect. We systematically developed an ELISA assay, optimizing different antigens and amplification steps, in serum and saliva from non‐hospitalised SARS‐CoV‐2‐infected subjects. Using trimeric spike glycoprotein, rather than nucleocapsid enabled detection of responses in individuals with low antibody responses. IgG1 and IgG3 predominate to both antigens, but more anti‐spike IgG1 than IgG3 was detectable. All antigens were effective for detecting responses in hospitalized patients. Anti‐spike IgG, IgA and IgM antibody responses were readily detectable in saliva from a minority of RT‐PCR confirmed, non‐hospitalized symptomatic individuals, and these were mostly subjects who had the highest levels of anti‐spike serum antibodies. Therefore, detecting antibody responses in both saliva and serum can contribute to determining virus exposure and understanding immune responses after SARS‐CoV‐2 infection.

Item Type: Article
Date Type: Publication
Status: Published
Schools: Medicine
Additional Information: This is an open access article under the terms of the Creative Commons Attribution License
Publisher: Wiley
ISSN: 0019-2805
Date of First Compliant Deposit: 17 May 2021
Date of Acceptance: 14 April 2021
Last Modified: 24 May 2023 00:32

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