LanCLs add glutathione to dehydroamino acids generated at phosphorylated sites in the proteome

Lai, Kuan-Yu, Galan, Sébastien R.G., Zeng, Yibo, Zhou, Tianhui Hina, He, Chang, Raj, Ritu, Riedl, Jitka, Liu, Shi, Chooi, K. Phin, Garg, Neha, Zeng, Min, Jones, Lyn H., Hutchings, Graham J. ORCID: https://orcid.org/0000-0001-8885-1560, Mohammed, Shabaz, Nair, Satish K., Chen, Jie, Davis, Benjamin G. and van der Donk, Wilfred A. 2021. LanCLs add glutathione to dehydroamino acids generated at phosphorylated sites in the proteome. Cell 184 (10) 10.1016/j.cell.2021.04.001

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Abstract

Enzyme-mediated damage repair or mitigation, while common for nucleic acids, is rare for proteins. Examples of protein damage are elimination of phosphorylated Ser/Thr to dehydroalanine/dehydrobutyrine (Dha/Dhb) in pathogenesis and aging. Bacterial LanC enzymes use Dha/Dhb to form carbon-sulfur linkages in antimicrobial peptides, but the functions of eukaryotic LanC-like (LanCL) counterparts are unknown. We show that LanCLs catalyze the addition of glutathione to Dha/Dhb in proteins, driving irreversible C-glutathionylation. Chemo-enzymatic methods were developed to site-selectively incorporate Dha/Dhb at phospho-regulated sites in kinases. In human MAPK-MEK1, such “elimination damage” generated aberrantly activated kinases, which were deactivated by LanCL-mediated C-glutathionylation. Surveys of endogenous proteins bearing damage from elimination (the eliminylome) also suggest it is a source of electrophilic reactivity. LanCLs thus remove these reactive electrophiles and their potentially dysregulatory effects from the proteome. As knockout of LanCL in mice can result in premature death, repair of this kind of protein damage appears important physiologically.

Item Type:	Article
Date Type:	Publication
Status:	Published
Schools:	Chemistry Cardiff Catalysis Institute (CCI)
Publisher:	Elsevier
ISSN:	0092-8674
Funders:	EPSRC, BBSRC and EU Horizon 2020
Date of First Compliant Deposit:	18 June 2021
Date of Acceptance:	31 March 2021
Last Modified:	04 May 2023 00:44
URI:	https://orca.cardiff.ac.uk/id/eprint/141986

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