Synthesis of silver nanoparticles using haloarchaeal isolate Halococcus salifodinae BK3

Srivastava, Pallavee, Bragança, Judith, Ramanan, Sutapa Roy and Kowshik, Meenal 2013. Synthesis of silver nanoparticles using haloarchaeal isolate Halococcus salifodinae BK3. Extremophiles 17 (5) , pp. 821-831. 10.1007/s00792-013-0563-3

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Abstract

Numerous bacteria, fungi, yeasts and viruses have been exploited for biosynthesis of highly structured metal sulfide and metallic nanoparticles. Haloarchaea (salt-loving archaea) of the third domain of life Archaea, on the other hand have not yet been explored for nanoparticle synthesis. In this study, we report the intracellular synthesis of stable, mostly spherical silver nanoparticles (AgNPs) by the haloarchaeal isolate Halococcus salifodinae BK3. The culture on adaptation to silver nitrate exhibited growth kinetics similar to that of the control. NADH-dependent nitrate reductase was involved in silver tolerance, reduction, synthesis of AgNPs, and exhibited metal-dependent increase in enzyme activity. The AgNPs preparation was characterized using UV–visible spectroscopy, XRD, TEM and EDAX. The XRD analysis of the nanoparticles showed the characteristic Bragg peaks of face-centered cubic silver with crystallite domain size of 22 and 12 nm for AgNPs synthesized in NTYE and halophilic nitrate broth (HNB), respectively. The average particle size obtained from TEM analysis was 50.3 and 12 nm for AgNPs synthesized in NTYE and HNB, respectively. This is the first report on the synthesis of silver nanoparticles by haloarchaea.

Item Type:	Article
Date Type:	Publication
Status:	Published
Schools:	Engineering
Publisher:	Springer
ISSN:	1431-0651
Funders:	Ministry of Earth Sciences
Date of Acceptance:	9 July 2013
Last Modified:	26 Jul 2021 16:15
URI:	https://orca.cardiff.ac.uk/id/eprint/142738

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