Cardiff University | Prifysgol Caerdydd ORCA
Online Research @ Cardiff 
WelshClear Cookie - decide language by browser settings

Antimicrobial activity of enacyloxin IIa and gladiolin against the urogenital pathogens 'Neisseria gonorrhoeae' and 'Ureaplasma' spp

Heath, N. L., Rowlands, R. S., Webster, G., Mahenthiralingam, E. ORCID: and Beeton, M. L. 2021. Antimicrobial activity of enacyloxin IIa and gladiolin against the urogenital pathogens 'Neisseria gonorrhoeae' and 'Ureaplasma' spp. Journal of Applied Microbiology 130 (5) , pp. 1546-1551. 10.1111/jam.14858

[thumbnail of jam.14858.pdf] PDF - Published Version
Available under License Creative Commons Attribution.

Download (146kB)


Aims To determine the antimicrobial activity of enacyloxin IIa and gladiolin against Neisseria gonorrhoeae and Ureaplasma spp. Methods and Results The Burkholderia polyketide antibiotics enacyloxin IIa and gladiolin were tested against 14 N. gonorrhoeae and 10 Ureaplasma spp. isolates including multidrug-resistant N. gonorrhoeae isolates WHO V, WHO X and WHO Z as well as macrolide, tetracycline and ciprofloxacin-resistant ureaplasmas. Susceptibility testing of N. gonorrhoeae was carried out by agar dilution, whereas broth micro-dilution and growth kinetic assays were used for Ureaplasma spp. The MIC range for enacyloxin IIa and gladiolin against N. gonorrhoeae was 0·015–0·06 mg l−1 and 1–2 mg l−1 respectively. The presence of resistance to front line antibiotics had no effect on MIC values. The MIC range for enacyloxin IIa against Ureaplasma spp. was 4–32 mg l−1 with a clear dose-dependent effect when observed using a growth kinetic assay. Gladiolin had no antimicrobial activity on Ureaplasma spp. at 32 mg l−1 and limited impact on growth kinetics. Conclusions Enacyloxin IIa and gladiolin antibiotics have antimicrobial activity against a range of antibiotic susceptible and resistant N. gonorrhoeae and Ureaplasma isolates. Significance and Impact of the Study This study highlights the potential for a new class of antimicrobial against pathogens in which limited antibiotics are available. Development of these compounds warrants further investigation in the face of emerging extensively drug-resistant strains.

Item Type: Article
Date Type: Publication
Status: Published
Schools: Biosciences
Additional Information: This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
Publisher: Wiley
ISSN: 1364-5072
Date of First Compliant Deposit: 30 July 2021
Date of Acceptance: 14 September 2020
Last Modified: 23 May 2023 18:02

Citation Data

Cited 4 times in Scopus. View in Scopus. Powered By Scopus® Data

Actions (repository staff only)

Edit Item Edit Item


Downloads per month over past year

View more statistics