Cardiff University | Prifysgol Caerdydd ORCA
Online Research @ Cardiff 
WelshClear Cookie - decide language by browser settings

Targeting human langerin promotes HIV-1 specific humoral immune responses

Kervevan, Jerome, Bouteau, Aurelie, Lanza, Juliane S., Hammoudi, Adele, Zurawski, Sandra, Surenaud, Mathieu, Dieudonne, Lydie, Bonnet, Marion ORCID:, Lefebvre, Cecile, Hocini, Hakim, Marlin, Romain, Gugin, Aurelie, Hersant, Barbara, Hermeziu, Oana, Menu, Elisabeth, Lacabaratz, Christine, Lelievre, Jean-Daniel, Zurawski, Gerard, Godot, Veronique, Henri, Sandrine, Igyarto, Botond Z., Levy, Yves and Cardinaud, Sylvain 2021. Targeting human langerin promotes HIV-1 specific humoral immune responses. PLoS Pathogens 17 (7) , e1009749. 10.1371/journal.ppat.1009749

[thumbnail of Kervevan et al journal.ppat.1009749-1.pdf] PDF - Published Version
Available under License Creative Commons Attribution.

Download (2MB)


The main avenue for the development of an HIV-1 vaccine remains the induction of protective antibodies. A rationale approach is to target antigen to specific receptors on dendritic cells (DC) via fused monoclonal antibodies (mAb). In mouse and non-human primate models, targeting of skin Langerhans cells (LC) with anti-Langerin mAbs fused with HIV-1 Gag antigen drives antigen-specific humoral responses. The development of these immunization strategies in humans requires a better understanding of early immune events driven by human LC. We therefore produced anti-Langerin mAbs fused with the HIV-1 gp140z Envelope (αLC.Env). First, we show that primary skin human LC and in vitro differentiated LC induce differentiation and expansion of naïve CD4+ T cells into T follicular helper (Tfh) cells. Second, when human LC are pre-treated with αLC.Env, differentiated Tfh cells significantly promote the production of specific IgG by B cells. Strikingly, HIV-Env-specific Ig are secreted by HIV-specific memory B cells. Consistently, we found that receptors and cytokines involved in Tfh differentiation and B cell functions are upregulated by LC during their maturation and after targeting Langerin. Finally, we show that subcutaneous immunization of mice by αLC.Env induces germinal center (GC) reaction in draining lymph nodes with higher numbers of Tfh cells, Env-specific B cells, as well as specific IgG serum levels compared to mice immunized with the non-targeting Env antigen. Altogether, we provide evidence that human LC properly targeted may be licensed to efficiently induce Tfh cell and B cell responses in GC

Item Type: Article
Date Type: Publication
Status: Published
Schools: Medicine
Subjects: Q Science > Q Science (General)
Additional Information: This is an open access article distributed under the terms of the Creative Commons Attribution License
Publisher: Public Library of Science
ISSN: 1553-7366
Date of First Compliant Deposit: 24 August 2021
Date of Acceptance: 24 June 2021
Last Modified: 11 May 2023 14:47

Citation Data

Cited 3 times in Scopus. View in Scopus. Powered By Scopus® Data

Actions (repository staff only)

Edit Item Edit Item


Downloads per month over past year

View more statistics