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Epigenetic differences in an identical genetic background modulate alternative splicing in A. thaliana

Chaudhary, Saurabh, Jabre, Ibtisaam and Syed, Naeem H. 2021. Epigenetic differences in an identical genetic background modulate alternative splicing in A. thaliana. Genomics 113 , pp. 3476-3486. 10.1016/j.ygeno.2021.08.006
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Abstract

How stable and temperature-dependent variations in DNA methylation and nucleosome occupancy influence alternative splicing (AS) remains poorly understood in plants. To answer this, we generated transcriptome, whole-genome bisulfite, and MNase sequencing data for an epigenetic Recombinant Inbred Line (epiRIL) of A. thaliana at normal and cold temperature. For comparative analysis, the same data sets for the parental ecotype Columbia (Col-0) were also generated, whereas for DNA methylation, previously published high confidence methylation profiles of Col-0 were used. Significant epigenetic differences in an identical genetic background were observed between Col-0 and epiRIL lines under normal and cold temperatures. Our transcriptome data revealed that differential DNA methylation and nucleosome occupancy modulate expression levels of many genes and AS in response to cold. Collectively, DNA methylation and nucleosome levels exhibit characteristic patterns around intron-exon boundaries at normal and cold conditions, and any perturbation in them, in an identical genetic background is sufficient to modulate AS in Arabidopsis.

Item Type: Article
Date Type: Publication
Status: Published
Schools: Biosciences
Publisher: Elsevier
ISSN: 0888-7543
Date of First Compliant Deposit: 25 October 2021
Date of Acceptance: 10 August 2021
Last Modified: 25 Oct 2021 08:13
URI: https://orca.cardiff.ac.uk/id/eprint/143972

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