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Dynamic shapes of the zygote and two-cell mouse and human

Graham, Chris F., Windsor, Shane, Ajduk, Anna, Trinh, Thanh, Vincent, Anna, Jones, Celine, Coward, Kevin, Kalsi, Dilraj, Zernicka-Goetz, Magdalena, Swann, Karl and Thomas, Adrian L. R. 2021. Dynamic shapes of the zygote and two-cell mouse and human. Biology Open 10 (12) , bio059013. 10.1242/bio.059013

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Abstract

Mouse zygote morphokinetics were measured during interphase, the mitotic period, cytokinesis, and two-cell stage. Sequences of rounder–distorted–rounder shapes were revealed, as were changing patterns of cross section area. A calcium chelator and an actin-disrupting agent inhibited the area changes that occurred between pronuclear envelope breakdown and cytokinesis. During cell division, two vortices developed in each nascent cell and they rotated in opposite directions at each end of the cell, a pattern that sometimes persisted for up to 10 h. Exchange with the environment may have been promoted by these shape and area cycles and persisting circulation in the cytoplasm may have a similar function between a cell's interior and periphery. Some of these movements were sporadically also seen in human zygotes with abnormal numbers of pronuclei and the two-cell stages that developed from these compromised human zygotes.

Item Type: Article
Date Type: Publication
Status: Published
Schools: Biosciences
Additional Information: This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0)
Publisher: The Company of Biologists
ISSN: 2046-6390
Date of First Compliant Deposit: 11 January 2022
Date of Acceptance: 10 November 2021
Last Modified: 13 Jan 2022 10:15
URI: https://orca.cardiff.ac.uk/id/eprint/146513

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