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Localization of P2X purinoceptor transcripts in the rat nervous system

Kidd, Emma Jane ORCID:, Grahames, C. B. A., Simon, J., Michel, A. D., Barnard, E. A. and Humphrey, P. P. A. 1995. Localization of P2X purinoceptor transcripts in the rat nervous system. Molecular Pharmacology 48 (4) , pp. 569-573.

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We used transcript-specific oligonucleotides to examine the localization in the rat nervous system of the corresponding mRNAs for the two P2X purinoceptor genes cloned recently from the rat vas deferens and PC12 cells. PC12 P2X purinoceptor mRNA was labeled in the olfactory tubercle, striatum, hypothalamus, hippocampus, dentate gyrus, amygdala, cortex, and cerebellum, whereas the vas deferens P2X purinoceptor-specific probes labeled the cerebellum and, at lower levels of expression, the striatum, hippocampus, and cortex. Both types of P2X purinoceptor transcript were found on cell bodies in the nodose and superior cervical ganglia. The presence of these two purinoceptor transcripts in the brain was confirmed by polymerase chain reaction. Two partial cDNAs, identical to sections of the PC12 or vas deferens P2X purinoceptor coding sequences, were amplified from neonatal brain and cerebellum poly(A)+ RNA, respectively. These findings are in broad agreement with earlier Northern blot studies on the PC12 P2X purinoceptor mRNA but differ from those for the vas deferens P2X purinoceptor mRNA, which had not previously been detected in adult brain. This difference is attributed to the low levels seen in the adult compared with the neonate and to the greater sensitivity of the methods used in the present study. The neonate medial habenula had low levels of transcripts for the PC12 but none for the vas deferens P2X purinoceptor. Because pharmacologically the recombinant PC12 P2X purinoceptor differs from the functional purinoceptor in the medial habenula, these results suggest the existence of other, unidentified, P2X purinoceptors in the rat nervous system.

Item Type: Article
Date Type: Publication
Status: Published
Schools: Pharmacy
Subjects: R Medicine > RM Therapeutics. Pharmacology
Publisher: American Society for Pharmacology and Experimental Therapeutics
ISSN: 1521-0111
Last Modified: 18 Oct 2022 13:39

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