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Extracellular flux analysis to investigate the impact of NF-κB on mitochondrial respiration in Colorectal Carcinoma (CRC)

Capece, Daria, Verzella, Daniela, Begalli, Federica, Bennett, Jason, D'Andrea, Daniel, Vecchiotti, Davide, Zazzeroni, Francesca and Franzoso, Guido 2021. Extracellular flux analysis to investigate the impact of NF-κB on mitochondrial respiration in Colorectal Carcinoma (CRC). NF-κB Transcription Factors: Methods and Protocols, Vol. 2366. Methods in Molecular Biology, Springer, pp. 293-303. (10.1007/978-1-0716-1669-7_18)
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Abstract

The reprogramming of cell metabolism is a hallmark of cancer. NF-κB transcription factors coordinate the host defense responses to stress, injury, and infection. They also play a central role in oncogenesis, at least in part by regulating cell metabolism and the adaptation to energy stress conditions in various types of cancer, such as colorectal carcinoma (CRC). Here, we describe the XF Cell Mito Stress Test methodology aimed at characterizing the metabolic and bioenergetic profile of CRC cells following the silencing of the essential NF-κB subunit, RelA. This methodology may also be applied to other cancers to reveal novel core vulnerabilities of malignant cells.

Item Type: Book Section
Date Type: Published Online
Status: Published
Schools: Medicine
MRC Centre for Neuropsychiatric Genetics and Genomics (CNGG)
Publisher: Springer
ISBN: 9781071616697
ISSN: 1064-3745
Date of First Compliant Deposit: 23 February 2022
Date of Acceptance: 9 July 2021
Last Modified: 02 Mar 2022 10:15
URI: https://orca.cardiff.ac.uk/id/eprint/147763

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