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Hepatitis A virus cellular receptor 1 (HAVcr-1) initiates prostate cancer progression in human cells via hepatocyte growth factor (HGF)-induced changes in junctional integrity

Telford, Emily A., Sanders, Andrew J. ORCID:, Owen, Sioned, Ruge, Fiona, Harrison, Gregory M., Jiang, Wen G. ORCID: and Martin, Tracey A. ORCID: 2022. Hepatitis A virus cellular receptor 1 (HAVcr-1) initiates prostate cancer progression in human cells via hepatocyte growth factor (HGF)-induced changes in junctional integrity. Biomolecules 12 (2) , 338. 10.3390/biom12020338

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Background: HAVcR-1 has been linked to cancer aetiology and may regulate junctional complexes, with its role in prostate cancer still unexplored. This study aims to investigate the expression of HAVcR-1 in prostate cancer samples and the exploration of the cellular/molecular impact of HAVcR-1. Methods: Levels of HAVcR-1 ectodomain in the serum of prostate cancer patients were compared to healthy controls, and assessed as the total protein and gene expression of HAVcR-1 and tissues sections. The manipulation of HAVcR-1 levels within prostate cancer cell lines determined changes in cell behaviour using in vitro cell models and barrier function assays. Protein/phosphoprotein levels were assessed using Western blotting. Results: Levels of HAVcR-1 ectodomain from serum were decreased in patients with prostate cancer. Ectodomain levels correlated with the Gleason score. Histologically, the total protein/gene expression of HAVcR-1 was overexpressed in prostate cancer. The overexpression of HAVcR-1 in prostate cancer cell lines resulted in key changes in cell behaviour and the phosphorylation of β-catenin with a concurrent decrease in membranous E-cadherin, increased nuclear β-catenin and increased cyclin D1 protein expression, which were associated with HGF-promoted changes in the barrier function. Conclusions: HAVcR-1 expression and ectodomain release coincides with the presence of prostate cancer; thus, indicating HAVcR-1 as a potential biomarker to aid in diagnostics, and implicating HAVcR-1 in the dysregulation of junctional complexes.

Item Type: Article
Date Type: Publication
Status: Published
Schools: Medicine
Additional Information: This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https:// 4.0/)
Publisher: MDPI
ISSN: 2218-273X
Funders: Cancer Research Wales, grant number Martin102014.
Date of First Compliant Deposit: 24 February 2022
Date of Acceptance: 17 February 2022
Last Modified: 01 Jun 2023 17:48

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