Tracking monocyte recruitment and macrophage accumulation in atherosclerotic plaque progression using a novel hCD68GFP/ApoE -/- reporter mouse - Brief report.

McNeill, Eileen, Iqbal, Asif J., Jones, Daniel, Patel, Jyoti, Coutinho, Patricia, Taylor, Lewis, Greaves, David R. and Channon, Keith M. 2017. Tracking monocyte recruitment and macrophage accumulation in atherosclerotic plaque progression using a novel hCD68GFP/ApoE -/- reporter mouse - Brief report. Arteriosclerosis, Thrombosis, and Vascular Biology 37 (2) , pp. 258-263. 10.1161/ATVBAHA.116.308367

PDF - Published Version Available under License Creative Commons Attribution. Download (815kB)

Abstract

Objective To create a model of atherosclerosis using green fluorescent protein (GFP)–targeted monocytes/macrophages, allowing analysis of both endogenous GFP+ and adoptively transferred GFP+ myeloid cells in arterial inflammation. Approach and Results hCD68GFP reporter mice were crossed with ApoE−/− mice. Expression of GFP was localized to macrophages in atherosclerotic plaques and in angiotensin II–induced aortic aneurysms and correlated with galectin 3 and mCD68 expression. Flow cytometry confirmed GFP+ expression in CD11b+/CD64+, CD11c+/MHC-IIHI, and CD11b+/F4/80+ myeloid cells. Adoptive transfer of GFP+ monocytes demonstrated monocyte recruitment to both adventitia and atherosclerotic plaque, throughout the aortic root, within 72 hours. We demonstrated the biological utility of hCD68GFP monocytes by comparing the recruitment of wild-type and CCR2−/− monocytes to sites of inflammation. Conclusions hCD68GFP/ApoE−/− mice provide a new approach to study macrophage accumulation in atherosclerotic plaque progression and to identify cells recruited from adoptively transferred monocytes.

Item Type:	Article
Date Type:	Publication
Status:	Published
Schools:	Medicine
Additional Information:	This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution, and reproduction in any medium, provided that the original work is properly cited
Publisher:	American Heart Association
ISSN:	1079-5642
Date of First Compliant Deposit:	1 August 2022
Date of Acceptance:	16 November 2016
Last Modified:	07 May 2023 02:48
URI:	https://orca.cardiff.ac.uk/id/eprint/148458

Citation Data

Cited 16 times in Scopus. View in Scopus. Powered By Scopus® Data

Actions (repository staff only)

Edit Item