CD161 expression defines new human γδ T cell subsets

Karunathilaka, Amali, Halstrom, Samuel, Price, Patricia, Holt, Michael, Lutzky, Viviana P., Doolan, Denise L., Kupz, Andreas, Bell, Scott C., Thomson, Rachel M., Miles, John J. and Ratnatunga, Champa N. 2022. CD161 expression defines new human γδ T cell subsets. Immunity and Ageing 19 (1) , 11. 10.1186/s12979-022-00269-w

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Abstract

γδ T cells are a highly versatile immune lineage involved in host defense and homeostasis, but questions remain around their heterogeneity, precise function and role during health and disease. We used multi−parametric flow cytometry, dimensionality reduction, unsupervised clustering, and self-organizing maps (SOM) to identify novel γδ T cell naïve/memory subsets chiefly defined by CD161 expression levels, a surface membrane receptor that can be activating or suppressive. We used middle-to-old age individuals given immune blockade is commonly used in this population. Whilst most Vδ1+subset cells exhibited a terminal differentiation phenotype, Vδ1− subset cells showed an early memory phenotype. Dimensionality reduction revealed eight γδ T cell clusters chiefly diverging through CD161 expression with CD4 and CD8 expression limited to specific subpopulations. Comparison of matched healthy elderly individuals to bronchiectasis patients revealed elevated Vδ1+ terminally differentiated effector memory cells in patients potentially linking this population with chronic proinflammatory disease.

Item Type:	Article
Date Type:	Publication
Status:	Published
Schools:	Medicine
Additional Information:	This article is licensed under a Creative Commons Attribution 4.0 International License
Publisher:	BioMed Central
ISSN:	1742-4933
Date of First Compliant Deposit:	25 March 2022
Date of Acceptance:	14 February 2022
Last Modified:	17 Jun 2023 02:13
URI:	https://orca.cardiff.ac.uk/id/eprint/148898

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