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Genipin delays corneal stromal enzymatic digestion

Donovan, Christopher, Koudouna, Elena ORCID:, Margo, Curtis E., Avila, Marcel Y. and Espana, Edgar M. 2021. Genipin delays corneal stromal enzymatic digestion. Translational Vision Science & Technology 10 (9) , 25. 10.1167/tvst.10.9.25

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Purpose: To evaluate the use of genipin in delaying enzymatic digestion of corneal stroma. Methods: Human corneal stromal tissue was treated with genipin, a known chemical crosslinker, and then along with control tissue was subjected to enzymatic digestion with collagenase. The effects of genipin treatment in retarding stromal digestion were analyzed with phase contrast microscopy, a protein quantification assay, second harmonic generation imaging, and transmission electron microscopy. Results: Genipin increased stromal resistance to enzymatic digestion when compared with untreated stroma. A morphologic analysis and protein quantification showed increased stromal resistance to enzymatic digestion once stromal tissue was treated with genipin. Second harmonic generation imaging revealed persistent fibrillar collagen signaling in genipin-treated tissue in contrast with untreated tissue suggesting that genipin retards collagenolysis. Conclusions: Genipin increases stromal resistance to enzymatic digestion in controlled experiments as demonstrated by protein quantification studies and through morphologic imaging. Translational Relevance: This study explores the novel use of genipin in delaying enzymatic stromal digestion. Delaying stromal melting in the setting of corneal infectious or autoimmune keratitis can potentially decrease clinical morbidity.

Item Type: Article
Date Type: Publication
Status: Published
Schools: Biosciences
Additional Information: This work is licensed under a Creative Commons Attribution 4.0 International License.
Publisher: ARVO
ISSN: 2164-2591
Date of First Compliant Deposit: 5 May 2022
Date of Acceptance: 23 July 2021
Last Modified: 05 Jan 2024 05:45

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