Photochemical approach for multiplexed biofunctionalisation of gallium arsenide

Santos Gomes, Bárbara and Masia, Francesco ORCID: https://orcid.org/0000-0003-4958-410X 2022. Photochemical approach for multiplexed biofunctionalisation of gallium arsenide. Journal of Colloid and Interface Science 625 , pp. 743-749. 10.1016/j.jcis.2022.06.071

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Abstract

The optoelectronic properties of gallium arsenide (GaAs) hold great promise in biosensing applications, currently being held back by the lack of methodologies reporting the spatially selective functionalisation of this material with multiple biomolecules. Here, we exploit the use of a photoreactive crosslinker - a diazirine derivative - for spatially selective covalent immobilisation of multiple bioreceptors on the GaAs surface. As a proof of principle we show the immobilisation of two proteins: neutravidin and endosulfine alpha protein. X-ray photoelectron spectroscopy results showed the presence of the biomolecules on the GaAs regions selectively exposed to ultraviolet light. The approach presented here is applicable to the covalent attachment of other biomolecules, paving the way for using GaAs as a platform for multiplexed biosensing.

Item Type:	Article
Date Type:	Publication
Status:	Published
Schools:	Biosciences
Additional Information:	This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
Publisher:	Elsevier
ISSN:	0021-9797
Date of First Compliant Deposit:	22 June 2022
Date of Acceptance:	17 June 2022
Last Modified:	10 Feb 2024 02:13
URI:	https://orca.cardiff.ac.uk/id/eprint/150620

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