In vivo magnetic resonance imaging of tumor protease activity

Haris, Mohammad, Singh, Anup, Mohammed, Imran ORCID: https://orcid.org/0000-0002-8412-0768, Ittyerah, Ranjit, Nath, Kavindra, Nanga, Ravi P.R., Debrosse, Catherine, Kogan, Feliks, Cai, Kejia, Poptani, Harish, Reddy, Damodar, Hariharan, Hari and Reddy, Ravinder 2014. In vivo magnetic resonance imaging of tumor protease activity. Scientific Reports 4 , 6081. 10.1038/srep06081

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Abstract

Increased expression of cathepsins has diagnostic as well as prognostic value in several types of cancer. Here, we demonstrate a novel magnetic resonance imaging (MRI) method, which uses poly-L-glutamate (PLG) as an MRI probe to map cathepsin expression in vivo, in a rat brain tumor model. This noninvasive, high-resolution and non-radioactive method exploits the differences in the CEST signals of PLG in the native form and cathepsin mediated cleaved form. The method was validated in phantoms with known physiological concentrations, in tumor cells and in an animal model of brain tumor along with immunohistochemical analysis. Potential applications in tumor diagnosis and evaluation of therapeutic response are outlined.

Item Type:	Article
Date Type:	Published Online
Status:	Published
Schools:	Optometry and Vision Sciences
Publisher:	Nature Research
ISSN:	2045-2322
Date of Acceptance:	24 July 2014
Last Modified:	04 Jun 2023 00:36
URI:	https://orca.cardiff.ac.uk/id/eprint/152691

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