Esterase screening using whole cells of Brazilian soil microorganisms

Mantovani, Simone M., Gonzaga De Oliveira, Luciana and Marsaioli, Anita J. 2010. Esterase screening using whole cells of Brazilian soil microorganisms. Journal of the Brazilian Chemical Society 21 (8) , pp. 1484-1489. 10.1590/S0103-50532010000800011

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Abstract

A miniaturized enzymatic assay using fluorescent probes to reveal esterase producing microorganisms was optimized and applied to screen 64 soil bacterial strains. The best results were validated using traditional non-fluorogenic assays with acetyl and propanoyl phenylethanol to confirm the miniaturized results. The most active microorganisms belong to the genus Bacillus showing esterase activity and good enantiomeric ratios for the resolution of phenylethanol derivatives (E > 30). Part of the microorganisms are kept in our laboratory in glycerol or freeze-dried and the best microorganisms will be deposited in the CBMAI/CPQBA/Unicamp culture collection.

Item Type:	Article
Date Type:	Published Online
Status:	Published
Schools:	Chemistry
Publisher:	Sociedade Brasileira de Química, SBQ
ISSN:	0103-5053
Last Modified:	14 Nov 2022 16:05
URI:	https://orca.cardiff.ac.uk/id/eprint/153697

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