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Direct protocol for ambient mass dpectrometry imaging on agar culture

Angolini, Célio Fernando F., Vendramini, Pedro Henrique, Araújo, Francisca D. S., Araújo, Welington L., Augusti, Rodinei, Eberlin, Marcos N. and Gonzaga De Oliveira, Luciana 2015. Direct protocol for ambient mass dpectrometry imaging on agar culture. Analytical Chemistry 87 (13) , pp. 6925-6930. 10.1021/acs.analchem.5b01538

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Herein we describe a new protocol that allows direct mass spectrometry imaging (IMS) of agar cultures. A simple sample dehydration leads to a thin solid agar, which enables the direct use of spray-based ambient mass spectrometry techniques. To demonstrate its applicability, metal scavengers siderophores were imaged directly from agar culture of S. wadayamensis, and well resolved and intense images were obtained using both desorption electrospray ionization (DESI) and easy ambient sonic-spray ionization (EASI) with well-defined selective spatial distributions for the free and the metal-bound molecules, providing clues for their roles in cellular metabolism.

Item Type: Article
Date Type: Publication
Status: Published
Schools: Chemistry
Publisher: American Chemical Society
ISSN: 0003-2700
Date of Acceptance: 11 June 2015
Last Modified: 14 Nov 2022 16:05

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