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Unveiling the bacterial sesquiterpenome of streptomyces sp. CBMAI 2042 discloses cyclases with versatile performances

Sachito, Douglas and Gonzaga De Oliveira, Luciana 2022. Unveiling the bacterial sesquiterpenome of streptomyces sp. CBMAI 2042 discloses cyclases with versatile performances. Journal of the Brazilian Chemical Society 33 (7) , pp. 734-742. 10.21577/0103-5053.20220013

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Abstract

Terpenes are the most abundant class of natural product that exist in nature. They possess a myriad of industrial applications including pharmaceutical, perfumery and flavors, bulk chemicals, and fuel. Intriguingly, until today, the vast majority of characterized terpenoids have been isolated from plants and fungi, and only in recent years bacteria were found to generate a representative reservoir of terpenoids molecules. Mining Streptomyces sp. CBMAI 2042 genome data has revealed the presence of five terpene cyclase genes. Chemical analysis of mycelium extract of this bacteria strain has unveiled at least 28 volatile terpenes molecules, where three encoding sesquiterpene cyclase (STC) genes are apparently responsible for their biosynthesis. The cyclic products obtained by incubation of these three purified recombinant STCs with farnesyl diphosphate (FPP) were analyzed by gas chromatography-mass spectrometry (GC-MS) and identified using the Van den Dool and Kratz equation

Item Type: Article
Date Type: Published Online
Status: Published
Schools: Chemistry
Publisher: Sociedade Brasileira de Química, SBQ
ISSN: 0103-5053
Date of First Compliant Deposit: 25 October 2022
Last Modified: 22 Nov 2022 15:32
URI: https://orca.cardiff.ac.uk/id/eprint/153718

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