In vivo study of monoamine oxidases using multisite intracerebral microdialysis

De Deurwaerdere, Philippe, Samb, Nouhad, El Boukhari, Hasna, Corne, Remi, Chagraoui, Abdeslam and Di Giovanni, Giuseppe 2022. In vivo study of monoamine oxidases using multisite intracerebral microdialysis. Methods in Molecular Biology: Monoamine Oxidase, Vol. 2558. Methods in Molecular Biology, Humana, New York: Springer, pp. 183-195. (10.1007/978-1-0716-2643-6_14)

Full text not available from this repository.

Abstract

The activity of monoamine oxidases (MAOs) in the brain is often associated with neurodegenerative diseases. The study of MAOs in vivo or ex vivo is generally performed using MAO inhibitors and rarely using substrates. We present a pharmacological approach using intracerebral microdialysis to study the activity of MAO in the striatum and the prefrontal cortex of rats. It consists of applying ascending concentrations of 3-methoxytyramine (3-MT) as a substrate via the probes and measuring the indirect product homovanillic acid generated by MAO activity. We present herein the methodologies comprising our in-house stereotaxic procedures in rats, the microdialysis perfusion system and the substrate application, and the neurochemical analysis of the samples.

Item Type:	Book Section
Date Type:	Published Online
Status:	Published
Schools:	Biosciences
Publisher:	Springer
Last Modified:	06 Dec 2022 11:00
URI:	https://orca.cardiff.ac.uk/id/eprint/154662

Citation Data

Cited 1 time in Scopus. View in Scopus. Powered By Scopus® Data

Actions (repository staff only)

Edit Item