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Nanosized copper(ii) oxide/silica for catalytic generation of nitric oxide from S-nitrosothiols

Kulyk, Kostiantyn, Azizova, Liana, Cunningham, James M., Mikhalovska, Lyuba, Borysenko, Mykola and Mikhalovsky, Sergey 2020. Nanosized copper(ii) oxide/silica for catalytic generation of nitric oxide from S-nitrosothiols. Journal of Materials Chemistry B: Materials for biology and medicine 8 (19) , pp. 4267-4277. 10.1039/D0TB00137F

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Abstract

Nitric oxide NO, mediates inflammatory and thrombotic processes and designing biomaterials capable of releasing NO in contact with biological tissues is considered to be a major factor aimed at improving their bio- and haemocompatibility and antibacterial properties. Their NO-releasing capacity however is limited by the amount of the NO-containing substance incorporated in the bulk or immobilised on the surface of a biomaterial. An alternative approach is based on the design of a material generating nitric oxide from endogenous NO bearing metabolites by their catalytic decomposition. It offers, at least in theory, an unlimited source of NO for as long as the material remains in contact with blood and the catalyst maintains its activity. In this paper we studied the catalytic properties of novel nanostructured CuO/SiO2 catalysts in generating NO by decomposition of S-nitrosoglutathione (GSNO) in vitro. CuO/SiO2 catalysts with different CuO loadings were synthesized by chemisorption of copper(II) acetylacetonate on fumed nanosilica followed by calcination. CuO content was controlled by a number of chemisorption–calcination cycles. Fourier-transform infrared spectroscopy and thermogravimetric analysis confirmed the formation of CuO/SiO2 nanoparticles (NPs) with particle size of CuO phase in the range from 71 to 88 nm. Scanning electron microscopy images revealed a uniform distribution of NPs without their sintering or agglomeration. All the materials of the CuO/SiO2 NP series exhibited NO-generating activity from GSNO confirmed by the Griess assay and by measuring the concentration of nitrite and nitrate anions in model solutions such as phosphate buffered saline and bovine serum. This activity is dependent on the material specific surface area and CuO exposure on the surface rather than CuO bulk content. The rate of NO production increased at higher initial concentration of the NO-bearing substrate studied in the range between 0.01 mM and 1.0 mM RSNO, which covers its physiological level. CuO/SiO2 NPs can be used to design polymers with NO generating properties at blood-biomaterial interface which are expected to have improved biocompatibility thus enhancing their potential for medical applications such as surgical tubing, peripheral venous catheters, auxiliary blood circulation devices and drug-eluting balloons.

Item Type: Article
Date Type: Publication
Status: Published
Schools: Dentistry
Publisher: Royal Society of Chemistry
ISSN: 2050-7518
Date of Acceptance: 6 April 2020
Last Modified: 23 Jan 2023 16:45
URI: https://orca.cardiff.ac.uk/id/eprint/155289

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