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Antimicrobial efficacy of endodontic irrigants from Azadirachta indica: an in vitro study

Dutta, Arindam ORCID: and Kundabala, Mala 2013. Antimicrobial efficacy of endodontic irrigants from Azadirachta indica: an in vitro study. Acta Odontologica Scandinavica 71 (6) , pp. 1594-1598. 10.3109/00016357.2013.780290

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Objective. This study analyzed the antimicrobial effect of five irrigants formulated from different parts of the tree Azadirachta indica (Neem) and compared with 2.5% sodium hypochlorite and 0.2% chlorhexidine gluconate through an agar diffusion test. Materials and methods. A clinical isolate of Candida albicans was innoculated on Sabourad Dextrose Agar and Enterococcus faecalis (ATCC 29212) on Sheep Blood Agar. Wells with 6 mm diameter were created in agar and 100 μL aliquiots of each irrigant were introduced to five different wells. After incubation, the largest uniform diameter of the inhibition zone was recorded. Results. The leaf extract of the tree and a mixture of the seed-bark powder dissolved in dimethyl sulfoxide were active against both organisms. The other neem-based irrigants, a leaf powder dissolved in dimethyl sulfoxide, aqueous bark decoction and neem oil, did not possess any antimicrobial efficacy. Sodium hypochlorite completely inhibited growth of C. albicans and the leaf extract had larger inhibition zones than chlorhexidine (p = 0.011) or the seed-bark irrigant (p = 0.008). Against E. faecalis, inhibition zones with chlorhexidine were the largest and differed significantly from sodium hypochlorite (p = 0.039), leaf extract (p = 0.008) and seed-bark irrigant (p = 0.011). Conclusions. Two neem irrigants displayed antimicrobial properties. The efficacy of the standard endodontic irrigants varied depending on the organisms tested. Clinical relevance: Neem-based endodontic irrigants may be formulated for clinical application.

Item Type: Article
Date Type: Published Online
Status: Published
Schools: Dentistry
Publisher: Taylor and Francis Group
ISSN: 0001-6357
Date of Acceptance: 19 February 2013
Last Modified: 23 Jan 2023 15:00

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