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Unsupervised analysis of FLIM-FRET data

Masia, Francesco ORCID: https://orcid.org/0000-0003-4958-410X, Borri, Paola ORCID: https://orcid.org/0000-0002-7873-3314, Dewitte, Walter ORCID: https://orcid.org/0000-0002-0606-1686 and Langbein, Wolfgang ORCID: https://orcid.org/0000-0001-9786-1023 2022. Unsupervised analysis of FLIM-FRET data. Presented at: 2022 IEEE Photonics Conference (IPC), Vancouver, Canada, 13-17 November. 2022 IEEE Photonics Conference (IPC). 10.1109/IPC53466.2022.9975544

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Abstract

We have developed an unsupervised method to analyze fluorescence lifetime imaging microscopy data based on non-negative matrix factorization. Additionally, by modelling the Förster resonant energy transfer process between donor and acceptor fluorophores, we retrieve the dynamics and the distribution of donor-acceptor pairs.

Item Type: Conference or Workshop Item (Paper)
Date Type: Published Online
Status: Published
Schools: Biosciences
ISBN: 978-1-6654-3487-4
ISSN: 2575-274X
Date of First Compliant Deposit: 24 January 2023
Date of Acceptance: 1 November 2022
Last Modified: 25 Feb 2023 02:30
URI: https://orca.cardiff.ac.uk/id/eprint/156201

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