A reporter of UV intensity delivered to the cytosol during photolytic uncaging

Brasen, Jens Christian, Dewitt, Sharon ORCID: https://orcid.org/0000-0001-8169-8241 and Hallett, Maurice Bartlett ORCID: https://orcid.org/0000-0001-8197-834X 2010. A reporter of UV intensity delivered to the cytosol during photolytic uncaging. Biophysical Journal 98 (7) , L25-L27. 10.1016/j.bpj.2009.12.4271

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Abstract

Photolytic uncaging of biologically-active molecules within cells is a powerful technique. However, the delivery of uncaging light into the cytosol can vary between cell types, individual cells of the same type, and different loci within an individual cell because of optical differences in absorbance and light-scattering properties of the cytoplasm. Here, we demonstrate a simple technique for monitoring the magnitude of cytosolic ultraviolet delivery during uncaging, which also leaves a quantitative and persistent record of this within the cells. The simple method shown here provides a much needed universal monitor of the delivery of ultraviolet light to molecules within the cytosol, providing a much needed parameter for the correct interpretation of uncaging experiments.

Item Type:	Article
Date Type:	Publication
Status:	Published
Schools:	Dentistry
Subjects:	Q Science > QH Natural history > QH426 Genetics R Medicine > R Medicine (General)
Publisher:	Cell Press
ISSN:	0006-3495
Funders:	Wellcome Trust (WT079962AIA), Danish Natural Science Research Council (Grant No. 272-06-0345)
Last Modified:	05 May 2023 08:28
URI:	https://orca.cardiff.ac.uk/id/eprint/15785

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