Cardiff University | Prifysgol Caerdydd ORCA
Online Research @ Cardiff 
WelshClear Cookie - decide language by browser settings

Collagen ultrastructural changes during stromal wound healing in organ cultured bovine corneas

Kamma-Lorger, Christina S., Boote, Craig ORCID:, Hayes, Sally ORCID:, Albon, Julie ORCID:, Boulton, Michael Edwin and Meek, Keith Michael Andrew ORCID: 2009. Collagen ultrastructural changes during stromal wound healing in organ cultured bovine corneas. Experimental Eye Research 88 (5) , pp. 953-959. 10.1016/j.exer.2008.12.005

Full text not available from this repository.


Corneal collagen ultrastructural changes occur during the healing process. The present study was designed to compare collagen ultrastructural changes after trephine wounding or flap creation. Bovine corneas were injured and maintained in organ culture for up to 4 weeks. Samples were removed from culture at 0, 1, 2, 3 and 4 weeks and snap frozen in liquid N2. X-ray scattering was used to measure changes in collagen interfibrillar spacing, intermolecular spacing and fibrillar diameter. Some samples were fixed in 10% Neutral Buffered Formalin solution and wax embedded for immunohistochemistry to monitor myofibroblast differentiation in corneal flaps. Swelling effects (i.e. changes in interfibrillar spacing) were more severe in trephined corneas than in those with stromal flaps. Collagen fibrillar diameter remained normal in the periphery of injured corneas, but increased significantly in areas within and around the wound in trephined samples and in the epithelial incision site in corneal flaps. Intermolecular spacing was unchanged in all samples. In the flaps, αSMA expression was only detected in an area adjacent to the epithelial plug, and cell numbers gradually increased during the culture. We conclude that stromal swelling is more rapid for trephine-wounded corneas than in stromal flaps, indicating that the intensity of the corneal healing response depends on the type of injury.

Item Type: Article
Date Type: Publication
Status: Published
Schools: Optometry and Vision Sciences
Subjects: R Medicine > R Medicine (General)
R Medicine > RE Ophthalmology
Uncontrolled Keywords: Wound healing; Cornea; Stromal flaps; Trephine wounds; Stromal swelling ; Organ culture; Collagen; X-ray scattering
Publisher: Elsevier
ISSN: 0014-4835
Last Modified: 18 Oct 2022 14:02

Citation Data

Cited 20 times in Scopus. View in Scopus. Powered By Scopus® Data

Actions (repository staff only)

Edit Item Edit Item