Morais, Tatiana P., Salamanca, Giulia, Vaz, Sandra H., Sebastião, Ana M., Bombardi, Cristiano, Crunelli, Vincenzo  ORCID: https://orcid.org/0000-0001-7154-9752 and Di Giovanni, Giuseppe
2026.
Developmental and cell type‐specific contributions of thalamic serotonin 2A receptors to absence seizures.
Epilepsia: Official journal of the International League Against Epilepsy
10.1002/epi.70272
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Abstract
Objective: Serotonin 2A receptors (5-HT2ARs) play a complex role in focal and generalized seizures due to their diverse cellular and regional distribution. Although systemic activation of 5-HT2ARs suppresses absence seizures (ASs) in Genetic Absence Epilepsy Rats From Strasbourg (GAERS) rats, the contribution of thalamic receptors and their cell-type specificity remains unclear. Here, we performed a developmental immunohistochemical analysis in the nucleus reticularis thalami (NRT) and the ventrobasal thalamic nucleus (VB) of GAERS rats to assess developmental alterations in 5-HT2AR expression and used genetic manipulation to determine whether the antiabsence effect of systemic 5-HT2AR activation depends on thalamocortical (TC) neurons or astrocytes. Methods: Double-immunofluorescence labeling of 5-HT2ARs with either γ-aminobutyric acid (GABA) or glial fibrillary acidic protein in adult GAERS rats was used to investigate the neuronal and astrocytic distribution of these receptors in the NRT and VB. In addition, [3H]GABA uptake and its modulation by 5-HT2AR activation were assessed in thalamic slices. Electroencephalographic and video recordings in freely moving GAERS were used to evaluate the effects of VB microinjection of TCB-2, a 5-HT2AR agonist, on ASs. Finally, the cellular mechanisms underlying these effects were investigated using selective shRNA-mediated knockdown of 5-HT2ARs in either TC neurons or astrocytes in the VB. Results: In the VB, at postnatal day (P) 25, 5-HT2ARs were mainly expressed in TC neurons and in the majority of the few GABAergic interneurons, whereas by P90 they were exclusively localized to TC neurons. In the NRT, neuronal expression increased from ~60% to nearly 100% over development. Astrocytic 5-HT2AR expression increased developmentally in the NRT but remained unchanged in the VB. GABA uptake was decreased in GAERS compared to Wistar rats and was not modified by 5-HT2AR activation. In vivo, intra-VB injection of TCB-2 reduced ASs; this effect was abolished by shRNA knockdown of 5-HT2ARs in TC neurons, but not in astrocytes. Significance: The developmental reorganization of thalamic 5-HT2AR signaling coincides with the expression of ASs, suggesting a contributory role. Our findings indicate that neuronal, but not astrocytic, thalamic 5-HT2ARs drive seizure modulation, identifying a potential therapeutic target.
| Item Type: | Article |
|---|---|
| Date Type: | Published Online |
| Status: | In Press |
| Schools: | Schools > Biosciences |
| Additional Information: | RRS policy applied |
| Publisher: | Wiley |
| ISSN: | 0013-9580 |
| Date of First Compliant Deposit: | 10 June 2026 |
| Last Modified: | 10 Jun 2026 10:00 |
| URI: | https://orca.cardiff.ac.uk/id/eprint/186899 |
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