Cai, Yinfan, Lu, Jingru, Dou, Kangjie, Li, Xiaonan, Ji, Liling, Orozco Ter Wengel, Pablo  ORCID: https://orcid.org/0000-0002-7951-4148, Hu, Tingli and Zhang, Baowei
2026.
A chromosome-level genome assembly of the Tristram’s Bunting (Emberiza tristrami).
Scientific Data
13
, 1012.
10.1038/s41597-026-07283-3
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Abstract
Emberiza tristrami is a migratory passerine within the genus Emberiza, a taxonomic group that has undergone rapid diversification despite broadly conserved avian karyotypes. Here, we present the first high-quality, chromosome-level reference genome for E. tristrami, generated using HiFi long reads combined with Hi-C sequencing. The final assembly spans 1.33 Gb, with a contig N50 of 22.42 Mb and a scaffold N50 of 64.94 Mb. Hi-C scaffolding anchored 1.23 Gb of assembled sequences into 40 pseudochromosomes, consistent with the known karyotype of the species. We annotated 17,401 protein-coding genes and identified 427.25 Mb of repetitive sequences, accounting for 32.09% of the genome. Genome completeness was assessed using BUSCO, recovering 99.3% of expected single-copy orthologs in genome mode and 91.7% in gene annotation mode, indicating a highly complete genome assembly and a high-quality set of annotated gene models. This chromosome-level genome provides a foundational resource for comparative analyses within Emberiza and other passerines, enabling investigations into chromosome evolution, repeat dynamics, and lineage-specific genomic features associated with rapid species radiation and ecological differentiation.
| Item Type: | Article |
|---|---|
| Date Type: | Published Online |
| Status: | Published |
| Schools: | Schools > Biosciences |
| Publisher: | Nature Research |
| ISSN: | 2052-4463 |
| Date of First Compliant Deposit: | 11 May 2026 |
| Date of Acceptance: | 14 May 2026 |
| Last Modified: | 06 Aug 2026 11:53 |
| URI: | https://orca.cardiff.ac.uk/id/eprint/186908 |
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