Antenatal maternal anaemia and infant brain structure: high-field (3 T) and ultra-low-field (64 mT) MRI findings from South Africa

Ringshaw, Jessica E., Zieff, Michael R., Bourke, Niall J., Casella, Chiara, Bradford, Layla E. and Jones, Derek K. ORCID: https://orcid.org/0000-0003-4409-8049 2026. Antenatal maternal anaemia and infant brain structure: high-field (3 T) and ultra-low-field (64 mT) MRI findings from South Africa. Brain Communications 8 (5) , fcag318. 10.1093/braincomms/fcag318

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Abstract

With the evolution of ultra-low-field MRI and the recognition of antenatal maternal anaemia as an important driver of altered neurodevelopment in toddlers and children, it is critical to determine whether these effects are detectable at ultra-low-field (64 mT) in infancy. The aim of this study was to assess the impact of antenatal maternal anaemia on infant brain structure across the first 2 years of life, using high-field (3 T) and ultra-low-field (64 mT) MRI. This neuroimaging substudy was embedded within Khula, an observational population-based birth cohort in South Africa. Pregnant women were enrolled antenatally and postnatally. Mother-child dyads (n = 394) were followed prospectively with a subsample attending neuroimaging at ∼3, 6, 12, 18 and 24 months of age. Anaemia was classified using World Health Organization thresholds, and neuroimaging data were processed using MiniMORPH. Linear mixed-effects models were used to investigate associations between antenatal maternal anaemia status and absolute regional infant brain volumes using high-field and ultra-low-field MRI. In repeated measures high-field (n = 195) and ultra-low-field (n = 341) infant neuroimaging subsamples, the prevalence of antenatal maternal anaemia was 28.24% (37/131) and 29.76% (61/205), respectively. Maternal anaemia in pregnancy was associated with altered child brain structure across both MRI systems, with group differences becoming detectable at ∼12 months. In the ultra-low-field subsample, infants born to anaemic mothers had 3.77% smaller intracranial volume (β = −0.24, P = 0.004) and 3.32% smaller putamen volumes (β = −0.18, P = 0.040) across the first 2 years of life. The interaction between antenatal maternal anaemia and age was significant for the caudate nucleus (β = −0.13, P = 0.038) and corpus callosum (β = −0.15, P = 0.007). Antenatal maternal anaemia was associated with 3.70% and 4.29% smaller caudate nucleus volumes at 18 and 24 months of age, respectively. Similarly, infants born to anaemic mothers had 4.39% smaller corpus callosum volumes by 12 months and 6.27% smaller corpus callosum volumes by 24 months. Postnatal child anaemia and antenatal maternal iron deficiency status were not associated with total or regional child brain volumes in the ultra-low-field subsample from this cohort. Maternal anaemia remained a robust predictor of volume differences in sensitivity analyses. This study is the first to demonstrate that the impact of maternal anaemia in pregnancy on child brain structure is detectable as early as infancy. The implications of this research are 2-fold: (i) informing the feasibility of ultra-low-field MRI in low- and middle-income countries and (ii) the timing and optimization of targeted recommendations for anaemia management in practice and policy.

Item Type:	Article
Date Type:	Published Online
Status:	Published
Schools:	Schools > Psychology Research Institutes & Centres > Cardiff University Brain Research Imaging Centre (CUBRIC)
Additional Information:	For full author list see article webpage https://doi.org/10.1093/braincomms/fcag318
Publisher:	Oxford University Press
ISSN:	2632-1297
Date of First Compliant Deposit:	14 September 2026
Date of Acceptance:	4 August 2026
Last Modified:	14 Sep 2026 15:15
URI:	https://orca.cardiff.ac.uk/id/eprint/189569

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