Induction of expression of a 14-3-3 gene in response to copper exposure in the marine alga, Fucus vesiculosus

Owen, Jennifer R., Morris, Ceri, Nicolaus, Beate, Harwood, John L. ORCID: https://orcid.org/0000-0003-2377-2612 and Kille, Peter ORCID: https://orcid.org/0000-0001-6023-5221 2012. Induction of expression of a 14-3-3 gene in response to copper exposure in the marine alga, Fucus vesiculosus. Ecotoxicology 21 (1) , pp. 124-138. 10.1007/s10646-011-0772-4

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Abstract

The macro-alga Fucus vesiculosus has a broad global and estuarine distribution and exhibits exceptional resistance to toxic metals, the molecular basis of which is poorly understood. To address this issue a cDNA library was constructed from an environmental isolate of F. vesiculosus growing in an area with chronic copper pollution. Characterisation of this library led to the identification of a cDNA encoding a protein known to be synthesised in response to toxicity, a full length 14-3-3 exhibiting a 71% identity to human/mouse epsilon isoform, 70–71% identity to yeast BMH1/2 and 95 and 71% identity to the Ectocarpus siliculosus 14-3-3 isoforms 1 and 2 respectively. Preliminary characterisation of the expression profile of the 14-3-3 indicated concentration- and time-dependent inductions on acute exposure of F. vesiculosus of copper (3–30 μg/l). Higher concentrations of copper (≥150 μg/l) did not elicit significant induction of the 14-3-3 gene compared with the control even though levels of both intracellular copper and the expression of a cytosolic metal chaperone, metallothionein, continued to rise. Analysis of gene expression within environmental isolates demonstrated up-regulation of the 14-3-3 gene associated with the known copper pollution gradient. Here we report for the first time, identification of a gene encoding a putative 14-3-3 protein in a multicellular alga and provide preliminary evidence to link the induction of this 14-3-3 gene to copper exposure in this alga. Interestingly, the threshold exposure profile may be associated with a decrease in the organism’s ability to control copper influx so that it perceives copper as a toxic response.

Item Type:	Article
Date Type:	Publication
Status:	Published
Schools:	Biosciences
Subjects:	G Geography. Anthropology. Recreation > GE Environmental Sciences Q Science > QH Natural history > QH301 Biology
Uncontrolled Keywords:	Phaeophyta; Copper; Toxicity; 14-3-3; Gene expression
Publisher:	Springer
ISSN:	0963-9292
Related URLs:	http://www.ncbi.nlm.nih.gov/pubmed/21909...
Last Modified:	19 Oct 2022 08:55
URI:	https://orca.cardiff.ac.uk/id/eprint/19480

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