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Induction of expression of a 14-3-3 gene in response to copper exposure in the marine alga, Fucus vesiculosus

Owen, Jennifer R., Morris, Ceri, Nicolaus, Beate, Harwood, John L. ORCID: and Kille, Peter ORCID: 2012. Induction of expression of a 14-3-3 gene in response to copper exposure in the marine alga, Fucus vesiculosus. Ecotoxicology 21 (1) , pp. 124-138. 10.1007/s10646-011-0772-4

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The macro-alga Fucus vesiculosus has a broad global and estuarine distribution and exhibits exceptional resistance to toxic metals, the molecular basis of which is poorly understood. To address this issue a cDNA library was constructed from an environmental isolate of F. vesiculosus growing in an area with chronic copper pollution. Characterisation of this library led to the identification of a cDNA encoding a protein known to be synthesised in response to toxicity, a full length 14-3-3 exhibiting a 71% identity to human/mouse epsilon isoform, 70–71% identity to yeast BMH1/2 and 95 and 71% identity to the Ectocarpus siliculosus 14-3-3 isoforms 1 and 2 respectively. Preliminary characterisation of the expression profile of the 14-3-3 indicated concentration- and time-dependent inductions on acute exposure of F. vesiculosus of copper (3–30 μg/l). Higher concentrations of copper (≥150 μg/l) did not elicit significant induction of the 14-3-3 gene compared with the control even though levels of both intracellular copper and the expression of a cytosolic metal chaperone, metallothionein, continued to rise. Analysis of gene expression within environmental isolates demonstrated up-regulation of the 14-3-3 gene associated with the known copper pollution gradient. Here we report for the first time, identification of a gene encoding a putative 14-3-3 protein in a multicellular alga and provide preliminary evidence to link the induction of this 14-3-3 gene to copper exposure in this alga. Interestingly, the threshold exposure profile may be associated with a decrease in the organism’s ability to control copper influx so that it perceives copper as a toxic response.

Item Type: Article
Date Type: Publication
Status: Published
Schools: Biosciences
Subjects: G Geography. Anthropology. Recreation > GE Environmental Sciences
Q Science > QH Natural history > QH301 Biology
Uncontrolled Keywords: Phaeophyta; Copper; Toxicity; 14-3-3; Gene expression
Publisher: Springer
ISSN: 0963-9292
Related URLs:
Last Modified: 19 Oct 2022 08:55

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