Riboflavin storage in earthworm chloragocytes and chloragocyte-derived eleocytes and its putative role as chemoattractant for immunocompetent cells.

Mazur, Agnieszka Irena, Klimek, Malgorzata, Morgan, Andrew John and Plytycz, Barbara 2011. Riboflavin storage in earthworm chloragocytes and chloragocyte-derived eleocytes and its putative role as chemoattractant for immunocompetent cells. Pedobiologia 54 (Suppl) , S37-S42. 10.1016/j.pedobi.2011.09.008

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Abstract

We have shown previously that riboflavin is stored in chloragosomes of chloragocyte-derived earthworm eleocytes what may have an adaptive value for worms vulnerable to soil-derived pathogen invasion. Thus, it is important to determine whether riboflavin stores are also present in earthworm species with low eleocyte counts, especially in the chloragocytes of their chloragogen tissue that surrounds the coelomic site of the intestine, and find out its putative roles in earthworm immunity. Experiments were performed on adult worms from 6 species. Freely floating coelomocytes (consisting of amoebocytes plus species-specific numbers of eleocytes) were extruded by mild electric shock and then chloragocytes from the same anaesthetized worm were mechanically detached into buffer. Both cell suspensions were analyzed by spectrofluorometry for riboflavin content. It turned out that riboflavin is stored in species-specific quantities in all investigated species. Riboflavin storage predominates in free coelomocytes of eleocyte-rich species (Eisenia andrei, Dendrobaena veneta, Allolobophora chlorotica) while in chloragocytes of species with few eleocytes (Lumbricus terrestris, L. rubellus, and Aporrectodea caliginosa). Upon a massive microbial impact, the coelomocytes (both amoebocytes and eleocytes) are involved in the formation of multicellular bodies encapsulating soil-derived pathogens, what is connected with enhanced coelomocyte mobility. Thus the second aim of the present investigation was to check if riboflavin can participate in coelomocyte accumulations. Tests performed in a 48-well chemotaxis chamber revealed that riboflavin behave as chemoattractant for coelomocytes of all investigated earthworm species, which may be one of mechanisms underpinning its immunomodulatory functions.

Item Type:	Article
Date Type:	Publication
Status:	Published
Schools:	Biosciences
Subjects:	G Geography. Anthropology. Recreation > GE Environmental Sciences Q Science > Q Science (General) Q Science > QH Natural history > QH301 Biology
Uncontrolled Keywords:	Earthworms; Riboflavin; Coelomocytes; Chloragocytes; Chemotaxis; Migratory activity
Additional Information:	from the 9th International Symposium on Earthworm EcologyXalapa, Veracruz, Mexico, 5th – 10th September 2010. Available online 19 September 2011.
Publisher:	Elsevier
ISSN:	0031-4056
Last Modified:	24 Mar 2017 03:16
URI:	https://orca.cardiff.ac.uk/id/eprint/21914

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