Characterisation of murine MICL (CLEC12A) and evidence for an endogenous ligand

Pyz, Elwira, Huysamen, Cristal, Marshall, Andrew S. J., Gordon, Siamon, Taylor, Philip Russel ORCID: https://orcid.org/0000-0003-0163-1421 and Brown, Gordon D. 2008. Characterisation of murine MICL (CLEC12A) and evidence for an endogenous ligand. European Journal of Immunology 38 (4) , pp. 1157-1163. 10.1002/eji.200738057

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Abstract

Inhibitory receptors are required for the control of cellular activation and they play essential roles in regulating homeostasis and immunity. We previously identified a human inhibitory C-type lectin-like receptor, MICL (CLEC12A), a heavily glycosylated monomer predominantly expressed on myeloid cells. Here we characterise the murine homolog of MICL (mMICL), and demonstrate that the receptor is structurally and functionally similar to the human orthologue (hMICL), although there are some notable differences. mMICL is expressed as a dimer and is not heavily glycosylated; however, like hMICL, the receptor can recruit inhibitory phosphatases upon activation, and is down-regulated on leukocytes following stimulation with selected TLR agonists. Using novel monoclonal antibodies, we demonstrate that, like the human receptor, mMICL is predominantly expressed by myeloid cells. However, mMICL is also expressed by B cells and CD8+ T cells in peripheral blood, and NK cells in the bone marrow. Finally, we show that mMICL recognises an endogenous ligand in a variety of murine tissues, suggesting that the receptor plays a role in homeostasis.

Item Type:	Article
Date Type:	Publication
Status:	Published
Schools:	Medicine Systems Immunity Research Institute (SIURI)
Subjects:	R Medicine > R Medicine (General)
Uncontrolled Keywords:	C-type lectin ; Endogenous ligand ; Inhibitory receptors ; Myeloid cell ; Myeloid inhibitory C-type lectin
Publisher:	Wiley Blackwell
ISSN:	0014-2980
Last Modified:	19 Oct 2022 09:52
URI:	https://orca.cardiff.ac.uk/id/eprint/22473

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