Complement component C9-dependent cytosolic free Ca2+ rise and recovery in neutrophils

Laffafian, Iraj, Davies, E. V., Campbell, Anthony Keith and Hallett, Maurice Bartlett ORCID: https://orcid.org/0000-0001-8197-834X 1995. Complement component C9-dependent cytosolic free Ca2+ rise and recovery in neutrophils. Cell Calcium 17 (4) , pp. 279-286. 10.1016/0143-4160(95)90074-8

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Abstract

The cytosolicfree Ca2+ concentration in rat neutrophils was determined by ratiometric fluorometry and imaging of Fura-2. Transient elevations of cytosolicfree Ca2+ concentration were provoked by addition of C9 to neutrophils pre-coated with C5b-8. The rate of rise of the cytosolicfree Ca2+ concentration was dependent upon the concentration of C9. These changes in cytosolicfree Ca2+ concentration occurred in the absence of cell lysis, since there was no release of Fura-2, and were the result of increased permeability to extracellular Ca2+. More than 96% of the rise in cytosolicfree Ca2+ generation by C9 was dependent upon the presence of extracellular Ca2+, but did not occur via channels which were inhibited by the Ca2+ channel blocker SKF96365. The decrease in the permeability of the membrane to Ca2+ after C9 was not triggered by the rise in cytosolicfree Ca2+. After attack by C9, individual neutrophils remained responsive to f-met-leu-phe, or further attack by C9, both producing Ca2+ transients. The recovery of the Ca2+ signal was consistent with the complement membrane attack complex generating a series of permeability thresholds in the plasma membrane. These data have implications for the understanding of the mechanisms underlying the inappropriate responsiveness of neutrophils at inflammatory sites.

Item Type:	Article
Date Type:	Publication
Status:	Published
Schools:	Medicine Pharmacy
Subjects:	Q Science > QD Chemistry Q Science > QR Microbiology R Medicine > RM Therapeutics. Pharmacology
Publisher:	Elsevier
ISSN:	0143-4160
Last Modified:	19 Oct 2022 09:52
URI:	https://orca.cardiff.ac.uk/id/eprint/22496

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