Kendall, Jonathan M., Badminton, Michael Norman, Sala-Newby, G. B., Campbell, Anthony Keith and Rembold, C. M. 1996. Recombinant apoaequorin acting as a pseudo-luciferase reports micromolar changes in the endoplasmic reticulum free Ca2+ of intact cells. Biochemical Journal 318 , pp. 383-387. |
Official URL: http://www.biochemj.org/bj/318/bj3180383.htm
Abstract
We describe a novel method to monitor the endoplasmic reticulum (ER) free Ca2+ in intact cells. Continuous perfusion of HeLa cells, expressing ER-targeted apoaequorin, with coelenterazine allowed the apoprotein to act as a pseudo-luciferase capable of reporting free Ca2+ from 0.1–100 µM. In intact HeLa cells, addition of ionomycin increased apoaequorin-generated light by 91%, indicating that resting ER free Ca2+ was approx. 2 µM. Agonist stimulation decreased the ER apoaequorin signal and proportionally increased cytosolic free Ca2+ consistent with agonist-induced release of Ca2+ from the ER.
Item Type: | Article |
---|---|
Date Type: | Publication |
Status: | Published |
Schools: | Medicine Pharmacy |
Subjects: | Q Science > QD Chemistry Q Science > QR Microbiology R Medicine > RM Therapeutics. Pharmacology |
Publisher: | Biochemical Society |
ISSN: | 0264-6021 |
Last Modified: | 05 Jun 2017 02:58 |
URI: | https://orca.cardiff.ac.uk/id/eprint/22508 |
Citation Data
Cited 25 times in Scopus. View in Scopus. Powered By Scopus® Data
Actions (repository staff only)
Edit Item |