Cardiff University | Prifysgol Caerdydd ORCA
Online Research @ Cardiff 
WelshClear Cookie - decide language by browser settings

The phototoxicity of aged human retinal melanosomes

Rozanowski, Bartosz, Cuenco, Joyceline, Davies, Sallyanne, Shamsi, Farukh Anis, Żądło, Andrzej, Dayhaw-Barker, Pierrette, Rozanowska, Malgorzata Barbara ORCID: https://orcid.org/0000-0003-2913-8954, Sarna, Tadeusz and Boulton, Michael Edwin 2008. The phototoxicity of aged human retinal melanosomes. Photochemistry and Photobiology 84 (3) , pp. 650-657. 10.1111/j.1751-1097.2007.00259.x

Full text not available from this repository.

Abstract

The purpose of this study was to determine whether an age-related increase in photoreactivity of human retinal melanosomes (MS) can cause phototoxicity to retinal pigment epithelium (RPE) cells. MS were isolated post mortem from young (20–30 years, young human melanosomes [YHMs]) and old (60–90 years, old human melanosomes [OHMs]) human eyes and from young bovine eyes (bovine melanosomes [BMs]). Confluent cultured ARPE-19 cells were fed equivalent numbers of OHMs or BMs and accumulated similar amounts of melanin as determined by electron paramagnetic resonance assay. Cells with and without MS were either maintained in the dark or exposed to blue light for up to 96 h and assessed for alterations in cell morphology, cell viability and lysosomal integrity. Incubation of cells in dark in the presence of internalized MS or irradiation of cells with blue light in the absence or presence of BMs did not significantly affect cell viability. However, exposures to blue light in the presence of OHMs resulted in abnormal cell morphology, up to ∼75% decrease in mitochondrial activity, loss of lysosomal pH and cell death. OHMs contained significantly less melanin than YHMs, supporting the hypothesis that melanin undergoes degradation during RPE aging. Our results demonstrate that aged MS can be phototoxic to human RPE cells and support a contributing role of MS in RPE aging and in the pathogenesis of age-related macular degeneration.

Item Type: Article
Date Type: Publication
Status: Published
Schools: Optometry and Vision Sciences
Subjects: R Medicine > RE Ophthalmology
ISSN: 0031-8655
Last Modified: 19 Oct 2022 09:57
URI: https://orca.cardiff.ac.uk/id/eprint/22748

Citation Data

Cited 51 times in Scopus. View in Scopus. Powered By Scopus® Data

Actions (repository staff only)

Edit Item Edit Item