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Spatial mapping of collagen fibril organisation in primate cornea - an X-ray diffraction investigation

Boote, Craig ORCID:, Hayes, Sally ORCID: and Meek, Keith Michael Andrew ORCID: 2004. Spatial mapping of collagen fibril organisation in primate cornea - an X-ray diffraction investigation. Journal of Structural Biology 146 (3) , pp. 359-367. 10.1016/j.jsb.2003.12.009

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New insights are presented into the collagenous structure of the primate cornea. Wide-angle X-ray diffraction was used to map the fibrillar arrangement and distribution of collagen over three common marmoset corneas. The maps provide a point of reference to help interpret data from pathological corneas or primate models of refractive surgery. The results herein disclose a circum-corneal annulus of highly aligned collagen, 0.5–1.5 mm wide, where the cornea and sclera fuse at the limbus; a feature similar to that observed in human tissue. As in humans, the annulus is not uniform, varying in width, fibril angular spread, and collagen density around its circumference. However, more centrally the marmoset cornea exhibits a preferred lamella orientation in which proportionally more fibrils are oriented along the superior–inferior corneal meridian. This observation is in striking contrast with the situation in human cornea, where there is an orthogonal arrangement of preferentially aligned fibrils. Investigation of a further 16 corneas confirmed that approximately 33% (±1%) (n=76) of fibrils in the central marmoset cornea lie within a 45° sector of the superior–inferior meridian. Implications for the mechanical and optical properties of the cornea are discussed.

Item Type: Article
Date Type: Publication
Status: Published
Schools: Optometry and Vision Sciences
Subjects: R Medicine > RE Ophthalmology
Uncontrolled Keywords: Cornea; Collagen; Lamellar structure; X-ray fibre diffraction; Primate; Marmoset monkey; Biomechanical; Light polarisation; Birefringence
Publisher: Elsevier
ISSN: 1047-8477
Last Modified: 20 Oct 2022 09:26

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