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Influence of titanium oxide and titanium peroxy gel on the breakdown of hyaluronan by reactive oxygen species

Taylor, G. C., Waddington, Rachel J. ORCID:, Moseley, Ryan ORCID:, Williams, K. R. and Embery, G. 1996. Influence of titanium oxide and titanium peroxy gel on the breakdown of hyaluronan by reactive oxygen species. Biomaterials 17 (13) , pp. 1313-1319. 10.1016/S0142-9612(96)80008-1

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The molecular events occuring at the interface between titanium and connective tissue were investigated in order to help explain the unique biocompatible properties of titanium implants and their successful osseointegration into bone tissue. In this study the influence of commercially pure titanium and titaniumperoxygels on the breakdown of the connective tissue component and serum derived factor, hyaluronan, by reactiveoxygenspecies (ROS), produced during the insertion of an implant in vivo, was examined. Hyaluronanbreakdown was monitored in vitro in the presence of a hydroxyl radical flux, generated in the presence and absence of titanium powder and discs. Parallel studies examined the breakdown of hyaluronan by hydroxyl radicals in the presence of a titaniumperoxygel, prepared by incubation of the titanium powder or discs in concentrated hydrogen peroxide. The hyaluronan degradation products were separated according to their hydrodynamic size by gel exclusion chromatography. Similarly, experiments were also performed examining the degradation of 2-deoxy-d-ribose by a hydroxyl radical flux in order to demonstrate the detrimental potential of the hydroxyl radicals and to provide a measure of the effectiveness of titanium and titaniumperoxygels as scavengers of ROS. Titanium reduced the harmful effects of the hydroxyl radicals on the breakdown of hyaluronan, presumably acting as a scavenger for the reactivespecies, possibly by absorbing them into its surface oxide layer, which spontaneously forms on the surface. In contrast, the formation of a titaniumperoxygel from the titanium powder or on the surface of titanium discs enhanced breakdown of both the hyaluronan chains and 2-deoxy-d-ribose. The implications of these findings with regards to the biocompatible nature of the titanium and the ability of these implants to successfully osseointegrate are discussed.

Item Type: Article
Date Type: Publication
Status: Published
Schools: Dentistry
Subjects: R Medicine > RK Dentistry
Uncontrolled Keywords: Titanium; reactiveoxygenspecies; hyaluronan; connective tissues; osseointegration
Publisher: Elsevier
ISSN: 0142-9612
Last Modified: 21 Oct 2022 08:50

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