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TGF-beta latency-associated peptides (LAPs) in human dentin matrix and pulp

Sloan, Alastair James ORCID:, Moseley, Ryan ORCID:, Dobie, K., Waddington, Rachel J. ORCID: and Smith, Amanda Jane 2002. TGF-beta latency-associated peptides (LAPs) in human dentin matrix and pulp. Connective Tissue Research 43 (2-3) , pp. 381-386. 10.1080/03008200290000916

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Transforming growth factor (TGF)- &#103 s in dentin matrix provide a pool of bioactive molecules, but association with latency-associated peptides (LAPs) may influence their activity. We investigated TGF- &#103 1, - &#103 2, and - &#103 3 LAP expression in sound and carious human teeth. Teeth were fixed and processed immediately following extraction prior to staining with rabbit polyclonal antibodies to the TGF- &#103 LAPs. A soluble dentin matrix fraction was prepared from dissected human dentin and sequential extraction of pulpal ECM was performed prior to purification. Fractions were Western blotted and probed with the LAP antibodies. All three LAPs were present in odontoblasts, cells of the pulp, and predentin; however, no staining of mineralized dentin matrix was seen. Similar patterns of expression were seen in carious tissue. Expression of TGF- &#103 LAPs in cells and pulpal matrix of healthy and carious teeth will be important in regulation of TGF- &#103 activity and may modulate the tissue response to injury.

Item Type: Article
Date Type: Publication
Status: Published
Schools: Dentistry
Subjects: R Medicine > RK Dentistry
Uncontrolled Keywords: Tgf-β, Odontoblasts, Dentin, Pulp, Immunohisto-chemistry
Publisher: Informa Healthcare
ISSN: 0300-8207
Last Modified: 08 Dec 2022 10:01

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