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Techniques and methodologies to study the ryanodine receptor at the molecular, subcellular and cellular level

Viero, Cedric, Thomas, Nia Lowri ORCID:, Euden, Joanne ORCID:, Mason, Sammy, George, Christopher H. and Williams, Alan John 2012. Techniques and methodologies to study the ryanodine receptor at the molecular, subcellular and cellular level. Islam, Md. Shahidul, ed. Calcium Signaling, Advances in Experimental Medicine and Biology, vol. 740. Dordrecht: Springer, pp. 183-215. (10.1007/978-94-007-2888-2_8)

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In excitable tissues, the ryanodine receptor Ca2+ release channel (RyR) protein complex regulates excitation-contraction coupling, exocytosis, gene expression and apoptosis. Defects in RyR function, in genetic or acquired pathologies, lead to massive disruptions of Ca2+ release that can be lethal. Therefore, RyR has emerged as a putative therapeutic target and an increasing number of RyR-targeting drugs are currently being tested. Nonetheless this large-size channel is still a mystery in terms of structure, which hinders full characterization of the properties of this central protein. This chapter is dedicated to the methods available to examine RyR structure and function. The aim of the article is to concentrate on contemporary methodologies rather than focusing overtly on the progress that has been achieved using these techniques. Here we review a series of reliable approaches that are routinely employed to investigate this channel. Technical limitations are discussed, and technological developments are presented. This work is not a handbook, but it can be used as a resource and a starting point for the investigation of RyR at different levels of resolution.

Item Type: Book Section
Date Type: Publication
Status: Published
Schools: Medicine
Subjects: R Medicine > R Medicine (General)
Uncontrolled Keywords: Ryanodine; Calcium channel; Lipid bilayer; Single channel recording; Cardiac myocyte; Transfection; HEK293; Sparks; Cryo-EM; Recombinant expression
Publisher: Springer
ISBN: 9789400728875
Related URLs:
Last Modified: 21 Oct 2022 10:40

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