HER2 gene amplification in breast cancer: a rogues' gallery of challenging diagnostic cases: UKNEQAS interpretation guidelines and research recommendations

Starczynski, Jane, Atkey, Neil, Connelly, Yvonne, O'Grady, Tony, Campbell, Fiona M., di Palma, Silvana, Wencyk, Peter, Jasani, Bharat, Gandy, Michael and Bartlett, J. M. S. 2012. HER2 gene amplification in breast cancer: a rogues' gallery of challenging diagnostic cases: UKNEQAS interpretation guidelines and research recommendations. American Journal of Clinical Pathology 137 (4) , pp. 595-605. 10.1309/AJCPATBZ2JFN1QQC

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Abstract

International and national guidelines highlight the importance of accuracy, reproducibility, and quality control of in situ hybridization (ISH) methods for testing breast carcinomas. However, few guidelines cover the reporting of ISH cases with “unusual” signal patterns, including, eg, heterogeneity and loss of chromosome enumeration probe or gene signals. These cases are, in fact, relatively frequent, and there is a need for developing evidence- or consensus-based reporting guidelines to ensure consistency of treatment. Following an audit of cases from a single center (including >1,700 cases) we show that approximately 10% of ISH results reflect unusual signal patterns. We illustrate the most common of these patterns and provide reporting guidelines for diagnosticians and recommendations for future research. Our goal is to ensure that in the future such “rogues” are reported in a consistent manner that, ultimately, will be supported by molecular and biochemical evidence.

Item Type:	Article
Date Type:	Publication
Status:	Published
Schools:	Medicine
Subjects:	Q Science > QH Natural history > QH426 Genetics R Medicine > R Medicine (General) R Medicine > RC Internal medicine > RC0254 Neoplasms. Tumors. Oncology (including Cancer)
Uncontrolled Keywords:	HER2 gene amplification; Breast cancer; In situ hybridization; Evidence-based reporting; Guidelines; Research
Publisher:	Lippincott, Williams & Wilkins
ISSN:	0002-9173
Last Modified:	18 Mar 2023 02:07
URI:	https://orca.cardiff.ac.uk/id/eprint/43105

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