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Apoptosis is observed in mesothelial cells after exposure to crocidolite asbestos

Berube, Kelly Ann ORCID:, Quinlan, T. R., Fung, H., Magae, J., Vacek, P., Taatjes, D. J. and Mossman, B. T. 1996. Apoptosis is observed in mesothelial cells after exposure to crocidolite asbestos. American Journal of Respiratory Cell and Molecular Biology 15 (1) , pp. 141-147. 10.1165/ajrcmb.15.1.8679218

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Asbestos causes protracted, dose-dependent increases in steady-state mRNA levels of the proto-oncogenes c-fos and c-jun, and AP-l DNA-binding activity in normal rat pleural mesothelial (RPM) cells (I). To determine the phenotypic end points of overexpression of these early response genes by asbestos, both cell proliferation and apoptosis were examined in confluent RPM cells exposed to a range of concentrations (1.25 to 10 p.g/cm' dish) of crocidolite asbestos for 24 and 48 h. Quantitation of RPM cells pulsed with 5-bromo-2'-deoxyuridine revealed that asbestos caused dose-dependent decreases in cells undergoing DNA synthesis. Decreases in cell proliferation were accompanied by dose-related increases in apoptosis using (l) terminal deoxynucleotidyl transferase-mediated deoxyuridine triphosphate nick-end labeling (i.e., ApopTag technique), (2) 4',6-diamidino-2-phenylindole cell staining, and (3) fluorescent-activated cell sorter after incorporation of propidium iodide. Less striking but significant dose-related increases in apoptosis were observed in RPM cells exposed to H20, (300 p.M), and no apoptosis was seen after exposure of cells to high concentrations (10 p.g/cm' dish) of glass beads. Our results are unique in that they demonstrate that asbestos induces apoptosis in mesothelial cells at concentrations eliciting increased expression of the proto-oncogenes c-fos and c-jun.

Item Type: Article
Date Type: Publication
Status: Published
Schools: Biosciences
Subjects: Q Science > QH Natural history > QH301 Biology
Q Science > QP Physiology
Publisher: American Thoracic Society
ISSN: 1044-1549
Last Modified: 24 Oct 2022 10:17

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