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Riboflavin content in autofluorescent earthworm coelomocytes is species-specific

Plytycz, Barbara, Homa, Joanna, Koziol, Beata, Rozanowska, Malgorzata Barbara and Morgan, Andrew John 2006. Riboflavin content in autofluorescent earthworm coelomocytes is species-specific. Folia Histochemica et Cytobiologica 44 (4) , pp. 275-280.

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We have recently shown that a large proproportion of earthworm coelomocytes exhibit strong autofluorescence in some species (Dendrobaena veneta, Allolobophora chlorotica, Dendrodrilus rubidus, Eisenia fetida, and Octolasion spp.), while autofluorescent coelomocytes are very scarce in representatives of Lumbricus spp. and Aporrectodea spp. Riboflavin (vitamin B2) was identified as a major fluorophore in Eisenia fetida coelomocytes. The main aim of the present experiments was to quantify riboflavin content in autofluorescent coelomocytes (eleocytes) from several earthworm species through a combination of flow cytometric and spectrofluorometric measurements. Spectrofluorometry of coelomocyte lysates showed that riboflavin was non-detectable in the coelomocytes of Aporrectodea spp. and Lumbricus spp., but was a prominent constituent of lysates from species with autofluorescent eleocytes. In the latter case, riboflavin content was the highest in E. fetida, followed by Octolasion spp. > A. chlorotica > D. rubidus. The riboflavin content of coelomocytes correlates positively with eleocyte autofluorescence intensity measured by flow cytometry and visible with fluorescence microscopy

Item Type: Article
Date Type: Publication
Status: Published
Schools: Biosciences
Optometry and Vision Sciences
Subjects: Q Science > QH Natural history > QH301 Biology
Publisher: Polish Histochemical and Cytochemical Society
ISSN: 0239-8508
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Last Modified: 04 Jun 2017 04:45

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